Name: chronic implantation of whole-cortical electrocorticographic array in the common marmoset
Uploaded: 2019-02-01T15:00:00
Description: Watch this Scientific Journal Video about Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset at JoVE.com

我们感谢尤里·希诺莫托提供动物护理、训练和清醒录音。ecog 阵列由 cir-tech (www.cir-tech.co.jp) 制造。此外, 我们要感谢 editage (www.editage.jp) 的英文编辑。这项工作得到了疾病综合神经技术 (brain/minds)、日本医学研究与发展署 (jp18dm0207001)、新科学倡议中心大脑科学项目的支持;cnsi)、国家自然科学研究所 (nins) (bs291004, m. k.) 和日本科学促进学会 (jpps) kakenhi (jph06034, m. k.)。

该协议已在6个普通虫上实施 (4名男性, 2名女性; 体重 = 3 0-470 克; 年龄 = 14-53个月)。所有程序都是根据《国家卫生研究院动物护理和使用准则》的建议进行的。该议定书得到了 riken 道德委员会的批准 (编号)。H28-2-221(3))。所有的外科手术都是在麻醉下进行的, 并尽一切努力尽量减少使用的动物数量和他们的不适。
1. 准备工作
<ol><li>获得每个大脑的结构磁共振图像 (mri)。这将用?...

<ol>
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A., Blumell, S., Kajikawa, Y., Hackett, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortical+connections+of+auditory+cortex+in+marmoset+monkeys:+lateral+belt+and+parabelt+regions.">Cortical connections of auditory cortex in marmoset monkeys: lateral belt and parabelt regions.</a> Anatomical Record. 295 (5), 800-821 (2012).</li><li>Kaas, J. H., Hackett, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subdivisions+of+auditory+cortex+and+processing+streams+in+primates.">Subdivisions of auditory cortex and processing streams in primates.</a> Proceedings of National Academy of Sciences of the United States of America. 97 (22), 11793-11799 (2000).</li><li>Ghahremani, M., Hutchison, R. M., Menon, R. S., Everling, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Frontoparietal+Functional+Connectivity+in+the+Common+Marmoset.">Frontoparietal Functional Connectivity in the Common Marmoset.</a> Cerebral Cortex. , (2016).</li><li>Belcher, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+Connectivity+Hubs+and+Networks+in+the+Awake+Marmoset+Brain.">Functional Connectivity Hubs and Networks in the Awake Marmoset Brain.</a> Frontiers in Integrative Neuroscience. 10, 9 (2016).</li><li>Mitchell, J. F., Leopold, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+marmoset+monkey+as+a+model+for+visual+neuroscience.">The marmoset monkey as a model for visual neuroscience.</a> Neuroscience Research. 93, 20-46 (2015).</li><li>Solomon, S. G., Rosa, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simpler+primate+brain:+the+visual+system+of+the+marmoset+monkey.">A simpler primate brain: the visual system of the marmoset monkey.</a> Frontiers in Neural Circuits. 8, 96 (2014).</li><li>Burman, K. J., Palmer, S. M., Gamberini, M., Rosa, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytoarchitectonic+subdivisions+of+the+dorsolateral+frontal+cortex+of+the+marmoset+monkey+(Callithrix+jacchus),+and+their+projections+to+dorsal+visual+areas.">Cytoarchitectonic subdivisions of the dorsolateral frontal cortex of the marmoset monkey (Callithrix jacchus), and their projections to dorsal visual areas.</a> Journals of Comparative Neurology. 495 (2), 149-172 (2006).</li><li>Bakola, S., Burman, K. J., Rosa, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+cortical+motor+system+of+the+marmoset+monkey+(Callithrix+jacchus).">The cortical motor system of the marmoset monkey (Callithrix jacchus).</a> Neuroscience Research. 93, 72-81 (2015).</li><li>Krubitzer, L. A., Kaas, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+organization+and+connections+of+somatosensory+cortex+in+marmosets.">The organization and connections of somatosensory cortex in marmosets.</a> Journal of Neuroscience. 10 (3), 952-974 (1990).</li><li>Miller, C. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Marmosets:+A+Neuroscientific+Model+of+Human+Social+Behavior.">Marmosets: A Neuroscientific Model of Human Social Behavior.</a> Neuron. 90 (2), 219-233 (2016).</li><li>Cox, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=AFNI:+software+for+analysis+and+visualization+of+functional+magnetic+resonance+neuroimages.">AFNI: software for analysis and visualization of functional magnetic resonance neuroimages.</a> Computers and Biomedical Research. 29 (3), 162-173 (1996).</li><li>Avants, B. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+reproducible+evaluation+of+ANTs+similarity+metric+performance+in+brain+image+registration.">A reproducible evaluation of ANTs similarity metric performance in brain image registration.</a> Neuroimage. 54 (3), 2033-2044 (2011).</li><li>Hashikawa, T., Nakatomi, R., Iriki, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+models+of+the+marmoset+brain.">Current models of the marmoset brain.</a> Neuroscience Research. 93, 116-127 (2015).</li></ol>

为了成功植入, 应在手术前后为动物提供充足的营养。短的操作时间对于优化动物的恢复也很重要。准备工作应在手术前至少一天完成。为了减少手术时间, 建议在终止的动物中插入电极阵列进行以前的开颅训练, 以达到其他实验目的。表 1显示了此协议的时间过程的示例。
我们在个案基础上修改了麻醉程序和术后治疗。在这个视频协议中, 这些动物被麻醉并通过?...

认知需要神经组合在广泛的大脑网络中的协调, 特别是在人类中发育良好并被认为参与更高认知行为的新皮层。然而, 新皮层如何实现这种认知行为是神经科学领域一个尚未解决的问题。薄、柔性电皮质 (ecog) 电极的最新发展使大规模皮质活动1的稳定记录能够传导。藤井和他的同事们已经开发出了一个完整的皮质 ecog 阵列的猕猴 2,3。该阵列持续覆盖几乎整个侧向皮层, 从枕极到颞叶和额叶杆, 并在一次拍摄中捕获全皮质神经活动。我们进一步开发了这个系统, 应用于普通的 4,5, 一个具有遗传可操纵性 6,7的新世界小猴子。与其他物种相比, 这种动物有几个优点。该物种的视觉、听觉、体感、运动和额叶皮质区域以前已经被绘制出来, 并报告有基本的同源组织到人类和猕猴的相同区域8, 9,10,11,12,13,14,15,16. 它们的大脑光滑, 大多数侧向皮质区域暴露在皮层表面, 这在猕猴中使用 ecog 是比较困难的。基于这些特点, 适合于电谱研究。此外, 表现出社会行为, 并被提出作为人类社会行为的候选模式.
该方案描述了在一个常见的马甲的皮层的整个外侧表面的 ecog 阵列硬膜外植入程序。它提供了一个机会来监测灵长类皮质神经科学的大规模皮质活动, 包括感官、运动、更高的认知和社会领域。

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Beaker (100 cc)</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Cotton ball</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Absorption triangles</td>
			<td>Fine Science Tools Inc.</td>
			<td>18105-03</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Cotton swab with fine tip</td>
			<td>Clean Cross Co., Ltd.</td>
			<td>HUBY340 BB-013</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Gauze</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Towel forceps</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Scalpel handle</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Needle Holder</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Iris Scissor</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Micro-Mosquito Forceps</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Adson, 1x2 teeth</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Bone Curette</td>
			<td></td>
			<td></td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Micro spatura</td>
			<td>Fine Science Tools Inc.</td>
			<td>10091-12</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Needle Holders, 12.5cm, Curved, Smooth Jaws</td>
			<td>World Precision Instruments</td>
			<td>14132</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Vessel Dilator, 12cm, 0.1mm tip</td>
			<td>Fine Science Tools Inc.</td>
			<td>18131-12</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Vessel Dilator, 12cm, 0.2 mm tip</td>
			<td>Fine Science Tools Inc.</td>
			<td>18132-12</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Fine-tipped rongeur</td>
			<td>Fine Science Tools Inc.</td>
			<td>16221-14</td>
			<td>Outocrave</td>
		</tr>
		<tr>
			<td>Manipurator of a stereotaxic frame</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Wrench for the manipurator</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Hand-made fixture for the connector</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Silicon cup for dental acril</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Silicon cup hlder</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Paintbrush</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Pencil</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Micro screw, 1.4 mm x 2.0 mm</td>
			<td>Nippon Chemical Screw Co., Ltd.</td>
			<td>PEEK/MPH-M1.4-L2</td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Screw driver for the micro screw</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Micromotor handpiece of a drill</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Stainless steel burr, 1.4 mm</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Stainless steel burr, 1.0 mm</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Drill bit, 1.2 mm</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
		<tr>
			<td>Rubber air blower</td>
			<td></td>
			<td></td>
			<td>Gas sterilization</td>
		</tr>
	</tbody>
</table>

chronic implantation of whole-cortical electrocorticographic array in the common marmoset

电皮质成像 (ecog) 允许监测来自大脑皮层的电场电位, 具有较高的时空分辨率。薄, 柔性 ecog 电极的最新发展使得能够进行大规模皮质活动的稳定记录。我们已经开发了一个全皮质 ecog 阵列的共同马甲。该阵列持续覆盖几乎整个侧向表面的皮质半球, 从枕杆到时间和额叶极点, 它捕获全皮质神经活动在一个镜头。该方案描述了在马甲大脑硬膜外空间的阵列的慢性植入程序。在 ecog 记录方面, 马麦子有两个优点, 一个是人类和猕猴解剖结构的同源组织, 包括额叶、顶叶和时间复合物。另一个优点是, 脑是耳脑, 含有大量的复合物, 在暴露在大脑表面的带有 ecog 的猕猴中更难进入。这些特征允许直接进入大脑表面下的大多数皮质区域。该系统提供了一个机会, 可以研究全球皮质信息处理的高分辨率, 在时间和毫米顺序的时间和毫米顺序在空间。

整个皮质 ecog 阵列可以同时捕获整个半球的神经元活动。图 4显示了清醒马甲内多个听觉区域的听觉诱发电位 (aep) 的例子。ecog 记录是在被动听力条件下进行的。每个都暴露在听觉刺激下, 其中包括具有20种频率的随机纯音调。然后, 我们通过平均 ecog 与一组音调对齐来计算 aep。从较低和较高的听觉区域观察到不同的波形, 这表明我们的 ecog 阵列的空?...

Watch this Scientific Journal Video about Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset at JoVE.com

Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset

常见马莫塞的全皮质电皮质阵列的慢性植入

我们已经开发了一个全皮质的电皮质阵列的共同马甲, 连续持续覆盖几乎整个侧表面的皮层, 从枕骨杆到颞叶和额叶杆。该方案描述了在马甲大脑硬膜外空间的阵列的慢性植入程序。

Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent ...

该协议演示了将电记录阵列植入非人类灵长类动物皮层。揭示灵长类动物转移神经科学的大规模信息处理。该技术的主要优点是，它提供了一个机会，监测大规模皮质活动与高空间时态分辨率。首先，通过头皮的中线做一个四厘米的皮肤切口。使用固化器将时间肌肉从头骨中分离出来，直到整个手术区域暴露。清除头骨表面的任何组织必要时用压力止血或骨蜡完全止血。用湿润的纱布包裹皮肤和肌肉的边缘。将阵列的正面边缘放在前极的边缘。使用铅笔标记计划颅骨切除术的区域，在头骨上缝开和打孔。将解剖显微镜置于手术区域。沿着标记 1 钻颅切除术。在钻孔时，在刀刃处吹气，以保持外科医生的清晰视野，一直切割骨骼，标记二，因为骨块仍将附着在中心杜拉上。慢慢地小心地，从一边抬起那块。用铲子剥下杜拉从骨片上取下骨尖，将骨包裹在湿润的纱布中，直到手术结束时将其返回到头骨。钻取标记为三和四，以便将电极分别插入轨道、正面和腹膜区域。钻孔在标记 5 上的缝隙，以便确认阵列是否正确插入。杜拉现在将暴露。用盐水清洗区域，必要时用压力血和明胶海绵止血。如果需要，清洁开放颅骨切除术的边缘，并标记六个缝隙，将参考电极放入其中。在头骨下插入一个铲子以保护杜拉物质，使用一毫米螺丝在连接器的每个阀杆周围四个点钻螺丝孔。并安装峰值螺钉作为锚点，将接头固定到头骨上。然后，使用平头钳来固定阵列，将心电图阵列插入硬膜外空间。将参考电极放在硬膜外空间中。然后将牙科丙烯酸应用到参考中。将接地电极放在颅面上。然后将牙科丙烯酸涂抹到接地电极上。将骨片放回位，将牙科丙烯酸涂抹在螺钉上，将接头和头部柱固定到头骨上。然后，使用 6 O 尼龙缝合线关闭前额和后部的皮肤。并使用皮肤闭合将皮肤固定到连接器的两侧。在这里，显示了从清醒的马莫塞特中多个听觉区域激发听觉电位的示例。在接触20种频率的随机纯音时，可以从低空和较高的听觉区域观察到不同的波形，表明心电图阵列的空间分辨率可以捕获不同皮质区域的不同信息处理。选项可以在马莫塞特大脑中病毒性地转导，因为电植入可实现长期慢性照片刺激和同时监测皮质反应。

Research

JoVE Journal

Neuroscience

Multielectrode Array Recordings of the Vomeronasal Epithelium

多电极阵列的犁鼻上皮录音

Understanding neural circuits requires methods to record from many neurons simultaneously. For in vitro studies, one currently available technology is ...

科研

神经科学

Surgical Implantation of Chronic Neural Electrodes for Recording Single Unit Activity and Electrocorticographic Signals

用于记录单机活动和Electrocorticographic信号的慢性神经电极植入手术

The success of long-term electrophysiological recordings often depends on the quality of the implantation surgery.  Here we provide useful information for ...

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

光纤植入慢性Optogenetic刺激脑组织

Elucidating patterns of neuronal connectivity has been a challenge for both clinical and basic neuroscience. Electrophysiology has been the gold standard ...

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

富氧运动器械中慢性硅探针的植入及海马位细胞的记录

An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced ...

Chronic Implantation of Multiple Flexible Polymer Electrode Arrays

多柔性聚合物电极阵列的慢性植入

Simultaneous recordings from large populations of individual neurons across distributed brain regions over months to years will enable new avenues of ...

Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset (Callithrix jacchus)

Stereotaxic Surgery for Implantation of Microelectrode Arrays in the Common Marmoset Callithrix jacchus

在普通马莫塞特中植入微电极阵列的立体外科（Callithrix jacchus）

Marmosets (Callithrix jacchus) are small non-human primates that are gaining popularity in biomedical and preclinical research, including the ...

Preparation of Peripheral Nerve Stimulation Electrodes for Chronic Implantation in Rats

大鼠慢性植入的外周神经刺激电极的制备

Peripheral nerve cuff electrodes have long been used in the neurosciences and related fields for stimulation of, for example, vagus or sciatic nerves. ...

Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice

使用腺相关病毒在小鼠中瞄准科菲林操纵的大脑皮层区域的电皮质记录

The use of electrocorticographic (ECoG) recordings in rodents is relevant to sleep research and to the study of a wide range of neurological conditions. ...

Direct Cannula Implantation in the Cisterna Magna of Pigs

直接插管植入猪的蓄水池

The glymphatic system is a waste clearance system in the brain that relies on the flow of cerebrospinal fluid (CSF) in astrocyte-bound perivascular spaces ...