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DOI :
10.3791/59038-v
March 14th, 2019
Chapters
0:04
Title
0:33
Designing the FRET Assay
1:12
Preparation of Cul1AMC•Rbx1, the FRET Donor Protein
4:42
Preparation of FlAsHCand1, the FRET Acceptor Protein
5:18
Testing and Confirmation of the FRET Assay
6:08
Measuring the Association Rate Constant
7:37
Measuring the Dissociation Rate Constant of Cul1•Cand1 in the Presence of Skp•F-box Protein
8:33
Results: Dynamics Of Protein Complexes
10:30
Conclusion
蛋白質蛋白質の相互作用、生物学的システムにとって重要と拘束運動の研究は、ダイナミクスとタンパク質複合体の機能に対する洞察を提供します。蛋白質の蛍光共鳴エネルギー移動ストップト フロー法を適用した複合体の速度論的パラメーターを定量化する手法について述べる。
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