January 28th, 2019
•Neuronal excitability can be modulated through a dynamic process of endo- and exocytosis of excitatory ionotropic glutamate receptors. Described here is an accessible, high-content assay for quantifying surface and internal receptor population pools.
Related Videos
Eye Movement Monitoring of Memory
Using an α-Bungarotoxin Binding Site Tag to Study GABA A Receptor Membrane Localization and Trafficking
A High Content Imaging Assay for Identification of Botulinum Neurotoxin Inhibitors
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation
Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved