Bacterial Liquid Culture and Induction of Functionalized Nanobody Expression
2:31
Purification of Functionalized Nanobodies
4:14
Validation of Functionalized Nanobody Expression
4:41
Uptake of Functionalized Nanobodies by Cultured Cells
8:03
Results: Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network
10:09
Conclusion
Transcript
This method enables the production and application of bacterial expressed, functionalised nano-bodies to analyze retrograde protein transport from the cell surface to intercellular compartments.In particular the Trans-Golgi network.The main advant
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Retrograde transport of proteins from the cell surface to the Golgi is essential to maintain membrane homeostasis. Here, we describe a method to biochemically analyze cell surface-to-Golgi transport of recombinant proteins using functionalized nanobodies in HeLa cells.