Name: use of in vivo single-fiber recording and intact dorsal root ganglion with attached sciatic nerve to examine the mechanism of conduction failure
Uploaded: 2019-08-27T13:00:00
Description: Watch this Scientific Journal Video about Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure at JoVE.com

这项工作得到了国家自然科学基金（31671089和81470060）和陕西省社会发展科学技术研究项目（2016SF-250）的资助。

目前的议定书遵循了《美国公共卫生服务关于人道照料和使用实验室动物的政策指南》，第四军医大学动物实验伦理委员会批准了该议定书。
1. 动物
<ol><li>将24只斯普拉格-道利大鼠（4-8周大）分成两组。通过一组14只大鼠和另一组10只大鼠用盐水治疗，在平面内注射100μL的CFA，生成完整的Freund辅助（CFA）模型。 注：所有动物都是从第四军医大学动物中心采集的。成年雄性?...

<ol>
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虽然最近的研究已经实现了DRG神经元在体内16的钙成像，从单个DRG受体进行体内贴片夹记录仍然极具挑战性。因此，在体内单纤维方法对疼痛场是持续重要的。本协议中的单光纤记录允许客观地观察传导失效现象，并且该技术与当前研究中开发的体外制剂相结合，可以检查临床前模型中的受体兴奋性变化。单光纤录制协议的三个步骤对于成功录制至关重要。首先，注意动物的麻?...

沿神经纤维的信息的正常传输保证了神经系统的正常功能。神经系统的异常功能也反映在神经纤维的电信号传输中。例如，通过比较干预应用1前后神经传导速度的变化，可以对中枢脱骨髓病变的脱骨髓程度进行分类。很难在细胞内记录神经纤维，除了特殊制剂，如鱿鱼巨型斧子2。因此，电生理活性只能通过单纤维的细胞外记录进行记录。单纤维记录作为传统的电生理方法之一，其历史比其他技术要长。然而，尽管该方法应用广泛，但掌握这种方法的电生理学家较少。因此，需要详细介绍单光纤记录的标准协议，以便进行适当的应用。
虽然各种贴片夹技术主导了现代电生理学研究，但单纤维记录在记录神经纤维的活动方面仍然发挥着不可替代的作用，尤其是纤维以神经纤维的外在感。位于背根结节（DRG）的感官细胞体。这里使用单纤维记录的优点是，体内纤维记录提供了很长的观察时间，能够记录临床前模型中对自然刺激的反应，而不会干扰细胞内环境3,4.
近二十年来，越来越多的研究研究了神经纤维5沿线的复杂功能，传导失败，被定义为沿着斧子的神经脉冲传递不成功的状态，存在于许多不同的外围神经6，7。在我们的调查中，传导失败的存在是沿着C纤维8调制持续感知输入的一种内在的自我抑制机制。在高乳腺4、9的条件下，这种传导失败显著减弱。因此，针对传导失败所涉及的因素可能代表神经病痛的新疗法。为了观察传导失败，应根据单纤维记录的连续放电尖峰记录和分析点火模式。
为了彻底了解传导失效的机制，有必要根据子极性解剖特性，确定斧子的传输特性，或者更确切地说，确定DRG神经元的膜特性。以前在这一领域的许多研究都进行了分离的DRG神经元10，11，这可能是不可行的研究传导失败，由于两个障碍。首先，在解散过程中使用各种机械和化学方法来释放DRG神经元，这可能导致不健康的细胞或改变神经元的表型/特性，并混淆结果。其次，附着的外周神经基本消除，传导失效现象在这些准备中无法观察到。因此，对具有附加神经的完整DRG神经元的准备已经改进，以避免上述障碍。

<table border="0" cellpadding="0" cellspacing="0" style="width:605px;" width="605">
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	<tbody>
		<tr height="42">
			<td height="42" style="height:43px;width:161px;">Instruments and software used in single fiber recording</td>
			<td style="width:148px;"></td>
			<td style="width:93px;"></td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Amplifier</td>
			<td style="width:148px;">Nihon kohden</td>
			<td style="width:93px;">MEZ-8201</td>
			<td style="width:203px;">Amplification of the electrophysiological signals</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:43px;width:161px;">Bioelectric amplifier monitor</td>
			<td style="width:148px;">ShangHai JiaLong Teaching instrument factory</td>
			<td style="width:93px;">SZF-1</td>
			<td style="width:203px;">Monitor firing process via sound which is transformed from physiological discharge signal</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Data acquisition and analysis system</td>
			<td style="width:148px;">CED</td>
			<td style="width:93px;">Spike-2</td>
			<td style="width:203px;">Software for data acquisition and analysis</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Electrode manipulator</td>
			<td style="width:148px;">Narishige</td>
			<td style="width:93px;">SM-21</td>
			<td style="width:203px;">Contro the movement of the electrode as required</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Hairspring tweezers</td>
			<td style="width:148px;">A.Dumont</td>
			<td style="width:93px;">5#</td>
			<td style="width:203px;">Separate the single fiber</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Isolator</td>
			<td style="width:148px;">Nihon kohden</td>
			<td style="width:93px;">SS-220J</td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="42">
			<td height="64" rowspan="2" style="height:64px;width:161px;">Memory oscilloscope</td>
			<td rowspan="2" style="width:148px;">Nihon kohden</td>
			<td rowspan="2" style="width:93px;">VC-9</td>
			<td style="width:203px;">Display recorded discharge during</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:203px;">experiment</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:161px;">Stereomicroscope</td>
			<td style="width:148px;">ZEISS</td>
			<td style="width:93px;">SV-11</td>
			<td style="width:203px;">Have clear observation when separate the local tissue and single fiber</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Stimulator</td>
			<td style="width:148px;">Nihon kohden</td>
			<td style="width:93px;">SEZ-7203</td>
			<td style="width:203px;">Delivery of the electrical stimuli</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Von Frey Hair</td>
			<td style="width:148px;">Stoelting accompany</td>
			<td style="width:93px;"></td>
			<td style="width:203px;">Delivery of the mechanical stimuli</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:161px;">Water bath</td>
			<td style="width:148px;">Scientz biotechnology Co., Ltd.</td>
			<td style="width:93px;">SC-15</td>
			<td style="width:203px;">Heating paroline to maintain at 37oC</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:161px;">Instruments and software used in patch clamp recording</td>
			<td style="width:148px;"></td>
			<td style="width:93px;"></td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="106">
			<td height="106" style="height:106px;width:161px;">Amplifier</td>
			<td style="width:148px;">Axon Instruments</td>
			<td style="width:93px;">Multiclamp 700B</td>
			<td style="width:203px;">Monitors the currents flowing through the recording electrode and also controls the stimuli by sending a signal to the electrode</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:161px;">Anti-vibration table</td>
			<td style="width:148px;">Optical Technology Co., Ltd.</td>
			<td style="width:93px;"></td>
			<td style="width:203px;">Isolates the recording system from vibrations induced by the environment</td>
		</tr>
		<tr height="127">
			<td height="127" style="height:127px;width:161px;">Camera</td>
			<td style="width:148px;">Olympus</td>
			<td style="width:93px;">TH4-200</td>
			<td style="width:203px;">See the neurons in bright field; the controlling software allows to take pictures and do live camera image to monitor the approach of the electrode to the cell</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Clampex</td>
			<td style="width:148px;">Axon</td>
			<td style="width:93px;">Clampex 9.2</td>
			<td style="width:203px;">Software for data acquisition and delivery of stimuli</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Clampfit</td>
			<td style="width:148px;">Axon</td>
			<td style="width:93px;">Clampfit 10.0</td>
			<td style="width:203px;">Software for data analysis</td>
		</tr>
		<tr height="66">
			<td height="66" style="height:66px;width:161px;">Electrode puller</td>
			<td style="width:148px;">Sutter</td>
			<td style="width:93px;">P-97</td>
			<td style="width:203px;">Prepare recording pipettes of about 2&#956;m diameter with resistance about 5 to 8 M&#937;</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Glass pipette</td>
			<td style="width:148px;">Sutter</td>
			<td style="width:93px;">BF 150-75-10</td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Micromanipulator</td>
			<td style="width:148px;">Sutter</td>
			<td style="width:93px;">MP225</td>
			<td style="width:203px;">Give a precise control of the microelectrode</td>
		</tr>
		<tr height="85">
			<td height="85" style="height:85px;width:161px;">Microscope</td>
			<td style="width:148px;">Olympus</td>
			<td style="width:93px;">BX51WI</td>
			<td style="width:203px;">Upright microcope equipped with epifluorescence for clearly observe the cells which would be patched</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Origin</td>
			<td style="width:148px;">Origin lab</td>
			<td style="width:93px;">Origin 8</td>
			<td style="width:203px;">Software for drawing picture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Perfusion Pump</td>
			<td style="width:148px;">BaoDing LanGe Co., Ltd.</td>
			<td style="width:93px;">BT100-1J</td>
			<td style="width:203px;">Perfusion of DRG in whole-cell patch clamp</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Other instruments</td>
			<td style="width:148px;"></td>
			<td style="width:93px;"></td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Electronic balance</td>
			<td style="width:148px;">Sartorius</td>
			<td style="width:93px;">BS 124S</td>
			<td style="width:203px;">Weighing reagent</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">pH Modulator</td>
			<td style="width:148px;">Denver Instrument</td>
			<td style="width:93px;">UB7</td>
			<td style="width:203px;">Adjust pH to 7.4</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Solutions/perfusion/chemicals</td>
			<td style="width:148px;"></td>
			<td style="width:93px;"></td>
			<td style="width:203px;"></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Calcium chloride</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">C5670</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:161px;">Chloralose</td>
			<td style="width:148px;">Shanghai Meryer Chemical Technology Co., Ltd.</td>
			<td style="width:93px;">M07752</td>
			<td style="width:203px;">Mixed solution for Anesthesia</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Collagenase</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">SLBQ1885V</td>
			<td style="width:203px;">Enzyme used for clearing the surface of DRG</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">D (+) Glucose</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">G7528</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Liquid Paraffin</td>
			<td style="width:148px;">TianJin HongYan Reagent Co., Ltd.</td>
			<td style="width:93px;"></td>
			<td style="width:203px;">Maintain fiber wetting</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Magnesium sulfate</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">M7506</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Potassium chloride</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">P3911</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Protease</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">62H0351</td>
			<td style="width:203px;">Enzyme used for clearing the surface of DRG</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Sodium bicarbonate</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">S5671</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Sodium chloride</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">S5886</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Sodium phosphate monobasic</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">S0751</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:161px;">Sucrose</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">S0389</td>
			<td style="width:203px;">Extracellular solution</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:161px;">Urethane</td>
			<td style="width:148px;">Sigma-Aldrich</td>
			<td style="width:93px;">U2500</td>
			<td style="width:203px;">Mixed solution for Anesthesia</td>
		</tr>
	</tbody>
</table>

use of in vivo single-fiber recording and intact dorsal root ganglion with attached sciatic nerve to examine the mechanism of conduction failure

单纤维记录是过去几十年中一种经典而有效的电生理技术，因为它在中枢和周围神经系统的神经纤维中有着特殊应用。这种方法特别适用于背根神经结膜（DRG），这是主要感觉神经元，表现出神经过程的伪单极结构。沿着斧子传递的动作电位的模式和特征在这些神经元中是可记录的。本研究使用体内单纤维记录来观察完全Freund的佐剂（CFA）处理大鼠的坐骨神经的传导失败。由于不能使用体内单纤维记录来研究底层机制，DRG神经元的贴片夹-记录在附加的坐骨神经的完整DRG的制剂上进行。这些记录揭示了在CFA处理的动物中，传导失败与DRG神经元后极化电位（AHP）的上升斜率之间的正相关关系。体内单纤维记录方案允许通过测量传导速度和监测某些疾病的神经纤维异常情况来对神经纤维进行分类。附加外周神经的完好 DRG 允许在大多数生理条件下观察 DRG 神经元的活动。最后，单纤维记录与完整DRG的电生理记录相结合是检查镇痛过程中传导失败作用的有效方法。

单光纤记录协议的结果取决于光纤解剖的质量。体内实验的动物必须在良好的状态，以保持神经躯干健康，便于解剖（见讨论部分的建议）。在许多情况下，在纤维上提供药物需要药物应用浴。图1说明了体内单纤维记录是如何操作的（图1A），并给出了一个经典记录，从CFA处理的动物的坐骨神经（图1B）。
以下实验调?...

Watch this Scientific Journal Video about Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure at JoVE.com

Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure

单纤维记录是一种有效的电生理技术，适用于中枢和周围神经系统。随着与附着的坐骨神经制备完整的DRG，检查传导失效的机制。这两种方案都有助于了解周围神经系统与疼痛的关系。

使用 Vivo 单纤维记录和带附骨神经的固定背道根结条，以检查传导失败机制

Single-fiber recording has been a classical and effective electrophysiological technique over the last few decades because of its specific application for ...

单纤维记录在记录神经纤维的活动中起着重要的作用。特别是活动传递外围感觉从接受场到神经元在后根结节。在光纤记录波中，提供持续时间长度，具有记录对自然刺激反应的能力，后来扰细胞间环境。在单个光纤记录中需要中子自动适合这种情况。治疗师称，坐骨神经是综合 DRG 测试的准备要求。演示情况的结果显示了几乎所有的神经细节在良好的，物理贴花阶段。第三，集成了整个 DRG 准备。要开始此程序，请准备在手术前对手术器械进行消毒。然后准备一到两升正常林格的细胞外溶液，并储存在四摄氏度，直到使用。要暴露坐骨神经躯干进行记录，首先，切开麻醉鼠的皮肤和大腿背部的肌肉。然后，沿着股骨二头肌进行钝解剖。用眼科剪刀和玻璃分离针小心分离坐骨神经躯干，使用林格溶液保持组织湿润。接下来，通过将皮肤缝入周围的槽，将动物固定到自制的金属箍上。稍微向上拉皮肤以建立一个液体浴。在近端暴露一厘米的坐骨神经躯干。在神经躯干下面放置一个棕色的小平台，以增强对比度，以便清楚地观察细神经躯干。随后，在神经干干顶部涂抹一些热液石蜡，以防止纤维表面干燥。如果神经干干周围有外化，请取出液体。要执行录制，请选择铂丝作为录制电极。吃，最后创建一个小钩子。之后，将电极连接到微操纵器上。在浴池中，在皮下组织旁边放置一个参考电极。分裂脊柱杜拉和皮亚母体，获得坐骨神经。在 25% 放大的立体显微镜下，拾起一个精细的分片，将轴的近似端悬挂在记录电极的挂钩上。使用机械模拟和热刺激确定单个非概念 c 纤维的接受场。如果神经纤维的燃烧对机械刺激和热水有反应，则视为多摩托、非概念性c纤维。接下来，在识别场的皮肤中插入两个针刺激电极，间隔为两毫米，用于传递电刺激。在示波器上显示作用电位的波形式，并采用信号采样速率为 20 千赫的 d 板计算机。然后，使用数据采集软件记录峰值，并在以后使用专业软件对其进行分析。要测量传导失败，请将不同频率的重复电极刺激传递到 c 光纤 60 秒。让纤维在刺激之间放松 10 分钟。然后，计算故障数与交付的重复刺激脉冲数的比率，乘以 100%以获得传导失败程度。要暴露背根结节，首先在 l4 到 l5 段从背面的中线切开皮肤。接下来，去除肌肉脊椎过程椎骨和横向过程使用骨隆格，并暴露脊髓NDRG身体。覆盖暴露的脊髓，NDRG，用普通林格的细胞外溶液浸泡棉花，以保持神经活动。停止出血，并删除血液，如有必要。随后，使用眼科剪刀，去除l4至s1骨骼结构上方的椎前体，以暴露 DRG 和连接的脊柱神经。在皮肤上切一下，露出大腿中间的坐骨神经。分离和断开坐骨神经从神经的远端，它进入肌肉内部。在切割前，在神经的尖端用手术线将神经躯干与手术线进行起立。然后，通过提升神经结扎点，将坐骨神经与底层结缔组织分离。从脊髓中去除dura，将DG从底层结缔组织分离，直到到达坐骨神经的相邻部分。因此，用附着的坐骨神经分离 DRG 的整个制备。要清除 DRG 的表面，在 4 倍放大时，请使用钳子小心去除 l4 到 l6 DRG 表面的脊柱杜拉。将具有附着坐骨神经的 DRG 放在含有一毫升混合酶的玻璃管中。在37摄氏度的水浴中消化15分钟。15分钟后，抬起手术线的端，将制剂移动到装满正常振铃器的细胞外溶液的盘中，以洗去酶。然后，将消化的 DRG 转移到装有氧振环器的细胞外溶液的容器中进行记录。要执行记录，请准备细胞内溶液，并将其存放在零摄氏度，直到使用。使用切片锚，稳定神经端，将神经端连接到吸力刺激电极。在 40 倍放大时，可视化并选择具有水 emersion 目标的 DRG 神经元。折叠电极，用细胞内溶液填充。将电极连接到支架上，并在管道中施加正压，最终电阻为 4 到 7 兆欧姆。接下来，将电极带到电池表面。然后，对移液器施加负压以形成密封。达到千兆孔密封后，将膜电位设置为负 60 毫伏，并建立所有细胞记录模式。随后，通过吸力电极向坐骨神经提供5至50赫兹的重复刺激，以筛查传导失败。测量 AHP 从基线到峰值的振幅和 80% AHP 持续时间。此图显示了大鼠对 10 赫兹电刺激的反应，从大鼠那里重新发射的原始连续记录。每 20 次扫描显示，并显示从上到下。插入显示具有代表性的操作潜力。以下是来自CFA注射大鼠的单c纤维的录音，以响应与上一个面板相同的刺激。此图显示了在控制条件下对五赫兹刺激的连续发射反应，或在 CFA 分级大鼠的小直径 DRG 神经元中对 ZD7288 的不同浓度进行连续记录。内集显示指定记录周期的扩展跟踪。黑点表示尖峰故障。AHP 在控制中显示了一个更大的上升斜率。在ZD7288应用的125微摩尔之后观察到较小的上升坡度。当一次单五录音时，我认为重要的是切纤维会保持动物良好的任何安全状态。和中子周围的微环境。单纤维记录的结合和与坐骨神经连接的完整的 DRG 的应用，增进了我们对与疼痛有关的周围神经系统的理解。

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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

培养成年的背根神经节神经元轴突再生的遗传研究

It is well known that mature neurons in the central nervous system (CNS) cannot regenerate their axons after injuries due to diminished intrinsic ability ...

科研

神经科学

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

Measuring Spinal Presynaptic Inhibition in Mice By Dorsal Root Potential Recording In Vivo

测量脊髓突触前抑制小鼠背根电位记录<em&gt;在体内</em

Presynaptic inhibition is one of the most powerful inhibitory mechanisms in the spinal cord. The underlying physiological mechanism is a depolarization of ...

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

In Vitro Modeling of Cancerous Neural Invasion: The Dorsal Root Ganglion Model

在癌神经侵袭的体外模型：背根神经节型号

One way that solid tumors disseminate is through neural invasion. This route is well-known in cancers of the head and neck, prostate, and pancreas. These ...

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

一种方法来提高背根神经节的对齐和髓鞘

Axon regeneration is a chaotic process due largely to unorganized axon alignment. Therefore, in order for a sufficient number of regenerated axons to ...

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

In Vivo Gene Transfer to Schwann Cells in the Rodent Sciatic Nerve by Electroporation

体内基因转移到雪旺细胞在啮齿动物坐骨神经电穿孔

The formation of the myelin sheath by Schwann cells (SCs) is essential for rapid conduction of nerve impulses along axons in the peripheral nervous ...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

背根神经节内注射和背根挤压伤为感官轴突再生模型

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each ...

Laminotomy for Lumbar Dorsal Root Ganglion Access and Injection in Swine

Laminotomy 对猪腰背根神经节通路和注射的治疗

Dorsal root ganglia (DRG) are anatomically well defined structures that contain all primary sensory neurons below the head. This fact makes DRG attractive ...

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

应用流式细胞仪分析单侧坐骨神经和背根神经节的免疫细胞

Nerve-resident immune cells in the peripheral nervous system (PNS) are essential to maintaining neuronal integrity in a healthy nerve. The immune cells of ...

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

哺乳动物轴突再生: 成年小鼠背根神经节体内电穿孔的研究

Electroporation is an essential non-viral gene transfection approach to introduce DNA plasmids or small RNA molecules into cells. A sensory neuron in the ...

Rapid Isolation of Dorsal Root Ganglion Macrophages

多尾根结鱼巨噬细胞的快速隔离

There are growing interests to study the molecular and cellular interactions among immune cells and sensory neurons in the dorsal root ganglia after ...