In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
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Results: Course of EAE and In Vivo Permeability
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Conclusion
Transcript
The methods presented here allow us to study the molecular mechanisms of immune cell migration across the brain barriers in experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. By investigating blood-brain barrier per
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Here, we present protocols to investigate impairment of the neurovascular unit during experimental autoimmune encephalomyelitis in vivo. We specifically address how to determine blood-brain barrier permeability and gelatinase activity involved in leukocyte migration across the glia limitans.Â