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DOI :
10.3791/59250-v
•
6:46 min
May 3rd, 2019
Chapters
0:04
Title
0:45
Preparation of the Multi-compartment Chips
1:05
Coating of Multi-compartment Chips for hSC-neurons
2:59
Seeding NSCs into the Multi-compartment Chip
4:05
Viral Fluorescent Labeling of hSC-neurons within the Chip
5:02
Results: Using Compartmentalized Microfluidic Chips to Isolate Neurons Differentiated from Human Stem Cells
6:16
Conclusion
该协议演示了使用分门分割的微流体芯片,注射成型在循环烯烃共聚物培养的神经元区别于人类干细胞。这些芯片是预先组装的,比传统的分块聚(二甲基硅氧烷)器件更容易使用。这里描述了多种常见的实验模式,包括病毒标记、流体隔离、轴切除术和免疫染色。
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