Name: measuring biophysical and psychological stress levels following visitation to three locations with differing levels of nature
Uploaded: 2019-06-19T13:00:00
Description: Watch this Scientific Journal Video about Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature at JoVE.com

这项研究部分资金来自印第安纳州布卢明顿印第安纳大学公共卫生学院赞助的教师研究资助计划(FRGP)。作者要感谢艾莉森·沃伊特博士和梅丽莎·佩奇博士的编辑协助和建设性意见。

本研究遵循印第安纳大学机构审查委员会人类研究保护计划的政策和指导方针。
1. 位置选择
<ol><li>根据不同的自然级别选择站点数 (n)。 注:我们选择了三个网站为我们的工作。A地块采用基于"自然性"水平的连续体,被认为是最自然的,由大约1,200英亩的林地山脊组成,毗邻湖泊,位于落叶林中。最常见的活动包括散步和观赏野生动物。站点 B 是一个占地 33 英亩的市政公园,设有?...

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本研究的目的是利用生物物理和心理工具,在娱乐访问后,确定压力的潜在变化,这些环境具有不同的自然水平。皮质醇和β-淀粉酶已被证明是心理压力水平的可靠指标。本研究中描述的淀粉酶测定程序已适应96井格式。当唾液中的淀粉酶水平高时,吸收性变化迅速发生。因此,限制一次分析的样本数量至关重要,因为一次可以分析的样本数量受每个井中 2.5 μL 的可添加速度的限制。在这项研究中,淀粉酶?...

压力水平升高长期以来与许多严重的健康问题有关,如心脏病、肥胖和心理障碍1、2、3。越来越多的研究表明,靠近或参观自然环境,如公园和未开发景观,可以产生显著的影响心理健康和减少压力水平1,4 5,6,7,8,9,10.对自然环境和压力水平影响的解释包括:(1) 自然环境为自然环境提供场所8、11 和 (2) 自然环境的访问者能够集中注意力对更多的非任务思维过程,从而减少注意力疲劳12。为了确定自然对减压的影响,本研究利用自我报告的心理压力(PSQ)和两个基于唾液的生物标志物,皮质醇和β-淀粉酶,在访问三个不同的娱乐场所后。这些地点因"自然"程度而异,包括荒野式环境、市政公园以及当地的健身和娱乐设施。
本研究旨在解决以下研究问题:(RQ 1)与所有三个部位(即自然、半自然、构建)进行比较时,唾液皮质醇和β-淀粉酶测量的生物物理应力水平是否有差异？(RQ2)与所有三个地点(即自然、半自然、建筑)相比,PSQ衡量的心理压力水平是否有差异(表现为四种结构:需求、忧虑、紧张和喜悦)？

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			<td height="53" style="height:54px;width:271px;">Cortisol Enzyme Immunoassay Kit</td>
			<td style="width:173px;">DetectX</td>
			<td style="width:119px;">K003-H1</td>
			<td style="width:677px;">The Cortisol Enzyme Immunoassay kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and culture media samples.</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:54px;width:271px;">Cryogenic Labels for Cryogenic Storage</td>
			<td style="width:173px;">Fisherbrand</td>
			<td style="width:119px;">5-910-A</td>
			<td style="width:677px;">Unique adhesive withstands extreme temperature</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:54px;width:271px;">Liquid Amylase (CNPG3) Reagent Set</td>
			<td style="width:173px;">Pointe Scientific</td>
			<td style="width:119px;">A7564</td>
			<td>For the quantitative kinetic determination of &#945;-amylase activity in human serum.</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:54px;width:271px;">Round Bottom 2mL Polypropylene Tubes with External Thread Cap</td>
			<td style="width:173px;">Greiner Bio-One</td>
			<td style="width:119px;">07-000-257</td>
			<td style="width:677px;">2.0 ml U-BTM Cryo.s self standing polypropylene sterilized</td>
		</tr>
		<tr height="53">
			<td height="53" style="height:54px;width:271px;">Synergy Multi-Mode Microplate Reader</td>
			<td style="width:173px;">BioTek</td>
			<td></td>
			<td style="width:677px;">It is a single-channel absorbance, fluorescence, and luminescence microplate reader that uses a dual-optics design to perform measurements of samples in a microplate format.</td>
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measuring biophysical and psychological stress levels following visitation to three locations with differing levels of nature

对自然环境的访问与心理压力的减轻有关。虽然大多数与压力相关的研究都依赖于自我报告格式,但越来越多的研究现在纳入了与生物压力相关的激素和催化剂,如皮质醇和β-淀粉酶,以测量压力水平。这里介绍的是一个协议,用于检查在访问三个不同地点后对生物物理和心理压力水平的影响,这些地点的性质水平不同。生物物理和自我报告的心理压力水平在进入选定地点后立即测量,并在访客离开现场之前进行测量。生物物理测量方法包括研究对象在进入三个研究地点之一时提供的1-2 mL唾液样本。根据现有文献的规定,唾液在访客在地点订婚后45分钟内收集。在收集唾液后,这些样本被标记并运送到生物实验室。皮质醇是本研究中感兴趣的生物物理变量,使用 ELISA 过程与 TECAN 板读取器进行测量。为了测量自我报告的压力,感知压力问卷 (PSQ) 报告担心、紧张、喜悦和感知需求的程度。数据在傍晚至傍晚在三个地点收集。当对所有三个设置进行比较时,通过生物标记和自我报告测量的应力水平在访问到最自然的设置后明显较低。

示例说明 利用配额抽样技术,这项研究从三个地点分别招募了35名访问者。在这项研究中共招募了105名受试者,包括63名男性和42名女性。从三个不同地点招募的访问者的平均年龄分别为25.9岁(网站A)、37.2岁(网站B)和28.8岁(网站C)。还记录了受试者访问选定三个地点的频率。对于站点 A 和站点 C,大多数受试者每周访问此站点一到三次。对于B站点的受试者,他们的访问频率平均为每周一至三...

Watch this Scientific Journal Video about Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature at JoVE.com

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

测量对三个自然水平不同的地点进行访问后的生物物理和心理压力水平

本文的目的是确定访问三种不同环境后压力水平的变化,并描述根据唾液皮质醇、β-淀粉酶和心理自我报告测量来确定压力水平的方法仪器。

Visitation to natural environments has been linked to psychological stress reduction. Although most stress-related research has relied on self-report ...

在这里，我们提出了一个协议，用于检查访问三个不同自然水平的位置后对生物物理和心理压力水平的影响。我们有兴趣了解一个自然度较高的网站是否会更有效地帮助参与者降低压力水平。除了使用自我报告问卷，使用这种技术的主要优点是，分析从参与者唾液样本收集的皮质醇和淀粉酶的水平可以让我们更准确地阅读和更好地了解我们的身体在参观娱乐场所后实际反应。演示这个程序的将是印第安纳大学教授霍塞瓦尔博士。首先介绍一下这个主题。向他们提供同意书，以签署解释研究的自愿性质、目的和程序。给主体一个红色的臂章，以便将来识别。通过唾液样本，在娱乐体验之前，并在体验结束后立即获得压力水平的生理和心理测量。使用被动流口水法收集一到两毫升唾液样本。指示受试者让唾液在嘴里汇集，然后流口水，从两英寸的吸管下进入冷冻瓶，直到收集到一毫升。使用分配的三位数 ID 号和字母标记样本，以指示数据收集的时间。然后，暂时将样品冷冻在充满干冰的类固醇泡沫盒中不超过两个小时。将标记的样品运送到实验室，并在零下 80 摄氏度下储存，直到分析。最后，使用液体淀粉酶试剂集来量化唾液样本中的α淀粉酶。然后，使用多模读卡器，在测定过程中，能够读取 405 纳米的光学密度，温度控制在 37 摄氏度。首先，获取皮质醇酶氨基酸试剂盒，以量化唾液样本中的皮质醇，一个分光光度计，用于读取450纳米的光学密度（OD）以及能够使用板读器的OD记录执行四个参数物流曲线拟合的软件。允许所有试剂平衡至 20 至 25 摄氏度，至少 30 分钟。使用去维化水稀释皮质醇测定缓冲液一至五，然后用去维水稀释洗涤缓冲液一至二十。接下来，在分析前将样品解冻在冰上。然后，用皮质醇测定缓冲液稀释样品1至10，并在制备后两小时内使用。为了准备标准，请给玻璃试管贴上一至七的标签。然后，将225微升的检测缓冲液移入1号管，将125微升缓冲液放入管二至七管中。接下来，添加25微升的皮质醇库存溶液到管一，和漩涡。最后，从1号管中取出125微升缓冲液，将其添加到二号管中，然后再次涡旋。结果显示，在访问自然环境 A 点后，唾液皮质醇水平显著下降。在站点 B 和 C 中未观察到皮质醇水平的显著变化。 此外，在受试者访问站点 C 后，发现对需求因素和担忧因素的显著减少，因此，在访问站点 C 后，Alpha 淀粉酶水平会显著增加。三个地点中的任何一个地点的因子张力没有显著变化，A站点和 B 站点报告因子喜悦情况显著增加。通常，关于减压的研究大部分依赖于自我报告问卷。在这项研究中，我们收集了参与者的唾液样本，以分析我们的身体在访问三个具有不同自然水平的部位后实际反应。在此可视化中，我们同时展示了实验室技术和填充数据收集的一个示例。这种技术使研究人员能够捕捉我们身体对不同环境的反应。在证实了我们的假设，自然环境可以对人类健康产生积极的影响。建议今后研究采用真正的实验设计，对不同自然环境或不同户外活动的压力水平进行生物物理测量。

Research

JoVE Journal

Behavior

Measuring Sensitivity to Viewpoint Change with and without Stereoscopic Cues

The speed and accuracy of object recognition is compromised by a change in viewpoint; demonstrating that human observers are sensitive to this ...

科研

行为学

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks

A set of behavioral tasks for assessing perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) is presented here ...

Mindfulness in Motion (MIM): An Onsite Mindfulness Based Intervention (MBI) for Chronically High Stress Work Environments to Increase Resiliency and Work Engagement

Mindfulness in Motion MIM: An Onsite Mindfulness Based Intervention MBI for Chronically High Stress Work Environments to Increase Resiliency and Work Engagement

正念的运动 （MIM）：一种基于现场干预正念（MBI）用于长期的高压力的工作环境，以增加弹性和工作投入

A pragmatic mindfulness intervention to benefit personnel working in chronically high-stress environments, delivered onsite during the workday, is timely ...

A Dual Task Procedure Combined with Rapid Serial Visual Presentation to Test Attentional Blink for Nontargets

的双任务过程结合快速系列视觉呈现到测试的注意闪烁Nontargets

When viewers search for targets in a rapid serial visual presentation (RSVP) stream, if two targets are presented within about 500 msec of each other, the ...

Wheel Running and Environmental Complexity as a Therapeutic Intervention in an Animal Model of FASD

车轮运行环境的复杂性在胎儿酒精中毒综合症的动物模型干预治疗

Aerobic exercise (e.g., wheel running (WR) extensively used in animal research) positively impacts many measures of neuroplastic potential in the brain, ...

A Within-subjects Experimental Protocol to Assess the Effects of Social Input on Infant EEG

一个被试内实验协议，以评估对婴幼儿脑社会投入的影响

Despite the importance of social interactions for infant brain development, little research has assessed functional neural activation while infants ...

Using Virtual Reality to Transfer Motor Skill Knowledge from One Hand to Another

利用虚拟现实技术将运动技能知识从一只手转移到另一个

As far as acquiring motor skills is concerned, training by voluntary physical movement is superior to all other forms of training (e.g. training by ...

Using Rapid Serial Visual Presentation to Measure Set-Specific Capture, a Consequence of Distraction While Multitasking

使用快速串行可视化演示来测量集特定捕获, 这是在多任务时分心的结果。

This method uses a rapid serial visual presentation (RSVP) paradigm to measure the cost of distraction when participants maintain multiple search goals. ...

Eye-tracking to Distinguish Comprehension-based and Oculomotor-based Regressive Eye Movements During Reading

眼动追踪在阅读中区分基于理解的和基于眼球的退行性眼球运动

Regressive eye movements are eye movements that move backwards through the text and comprise approximately 10-25% of eye movements during reading. As ...

A View of Their Own: Capturing the Egocentric View of Infants and Toddlers with Head-Mounted Cameras

自己的观点: 用头摄像头捕捉婴幼儿的自我中心观

Infants and toddlers view the world, at a basic sensory level, in a fundamentally different way from their parents. This is largely due to biological ...