Name: modified single-loop reconstruction for pancreaticoduodenectomy
Uploaded: 2019-09-28T12:30:00
Description: Watch this Scientific Journal Video about Modified Single-Loop Reconstruction for Pancreaticoduodenectomy at JoVE.com

Non applicable.

Ce protocole se concentre sur la phase de reconstruction de la DP à l'aide de mSLR et suit les directives du comité d'éthique de la Ruhr-Université de Bochum, Allemagne.
REMARQUE : Les patients présentant une tumeur résécable de la tête pancréatique, de la région papillaire ou de la pancréatite calcifiante chronique réfractaire grave ont subi. La résectabilité était présente si la tumeur avait moins de 50 % de contact aux artères péripancréatiques et n'a pas subi infiltrer l...

<ol>
	<li>Cameron, J. L., He, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+thousand+consecutive+pancreaticoduodenectomies.">Two thousand consecutive pancreaticoduodenectomies.</a> Journal of American College of Surgeons. 220 (4), 530-536 (2015).</li><li>Keck, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancreatogastrostomy+Versus+Pancreatojejunostomy+for+RECOnstruction+After+PANCreatoduodenectomy+(RECOPANC,+DRKS+00000767):+Perioperative+and+Long-term+Results+of+a+Multicenter+Randomized+Controlled+Trial.">Pancreatogastrostomy Versus Pancreatojejunostomy for RECOnstruction After PANCreatoduodenectomy (RECOPANC, DRKS 00000767): Perioperative and Long-term Results of a Multicenter Randomized Controlled Trial.</a> Annals of Surgery. 263 (3), 440-449 (2016).</li><li>Deng, L. H., Xiong, J. J., Xia, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolated+Roux-en-Y+pancreaticojejunostomy+versus+conventional+pancreaticojejunostomy+after+pancreaticoduodenectomy:+a+systematic+review+and+meta-analysis.">Isolated Roux-en-Y pancreaticojejunostomy versus conventional pancreaticojejunostomy after pancreaticoduodenectomy: a systematic review and meta-analysis.</a> Journal of Evidence Based Medicine. 10 (1), 37-45 (2017).</li><li>Klaiber, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Meta-analysis+of+complication+rates+for+single-loop+versus+dual-loop+(Roux-en-Y)+with+isolated+pancreaticojejunostomy+reconstruction+after+pancreaticoduodenectomy.">Meta-analysis of complication rates for single-loop versus dual-loop (Roux-en-Y) with isolated pancreaticojejunostomy reconstruction after pancreaticoduodenectomy.</a> British Journal of Surgery. 102 (4), 331-340 (2015).</li><li>Eshmuminov, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+review+and+meta-analysis+of+postoperative+pancreatic+fistula+rates+using+the+updated+2016+International+Study+Group+Pancreatic+Fistula+definition+in+patients+undergoing+pancreatic+resection+with+soft+and+hard+pancreatic+texture.">Systematic review and meta-analysis of postoperative pancreatic fistula rates using the updated 2016 International Study Group Pancreatic Fistula definition in patients undergoing pancreatic resection with soft and hard pancreatic texture.</a> HPB (Oxford). 20 (11), 992-1003 (2018).</li><li>Ke, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+prospective,+randomized+trial+of+Roux-en-Y+reconstruction+with+isolated+pancreatic+drainage+versus+conventional+loop+reconstruction+after+pancreaticoduodenectomy.">A prospective, randomized trial of Roux-en-Y reconstruction with isolated pancreatic drainage versus conventional loop reconstruction after pancreaticoduodenectomy.</a> Surgery. 153 (6), 743-752 (2013).</li><li>El Nakeeb, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolated+Roux+loop+pancreaticojejunostomy+versus+pancreaticogastrostomy+after+pancreaticoduodenectomy:+a+prospective+randomized+study.">Isolated Roux loop pancreaticojejunostomy versus pancreaticogastrostomy after pancreaticoduodenectomy: a prospective randomized study.</a> HPB (Oxford). 16 (8), 713-722 (2014).</li><li>Tani, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Randomized+clinical+trial+of+isolated+Roux-en-Y+versus+conventional+reconstruction+after+pancreaticoduodenectomy.">Randomized clinical trial of isolated Roux-en-Y versus conventional reconstruction after pancreaticoduodenectomy.</a> British Journal of Surgery. 101 (9), 1084-1091 (2014).</li><li>Machado, M. C., da Cunha, J. E., Bacchella, T., Bove, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+technique+for+the+reconstruction+of+the+alimentary+tract+after+pancreatoduodenectomy.">A modified technique for the reconstruction of the alimentary tract after pancreatoduodenectomy.</a> Surgery Gynecology Obstetrics. 143 (2), 271-272 (1976).</li><li>Aghalarov, I., Herzog, T., Uhl, W., Belyaev, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+single-loop+reconstruction+after+pancreaticoduodenectomy+reduces+severity+of+postoperative+pancreatic+fistula+in+high-risk+patients.">A modified single-loop reconstruction after pancreaticoduodenectomy reduces severity of postoperative pancreatic fistula in high-risk patients.</a> HPB (Oxford). 20 (7), 676-683 (2018).</li><li>Marangoni, G., Morris-Stiff, G., Deshmukh, S., Hakeem, A., Smith, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modern+approach+to+teaching+pancreatic+surgery:+stepwise+pancreatoduodenectomy+for+trainees.">A modern approach to teaching pancreatic surgery: stepwise pancreatoduodenectomy for trainees.</a> Journal of Gastrointestinal Surgery. 16 (8), 1597-1604 (2012).</li><li>. Pyloruserhaltende Duodenohemipankreatektomie nach Traverso-Longmire mit T-Drainage (pp-Whipple) Available from: <a target="_blank" href="https://www.webop.de/Allgemein-und-Viszeralchirurgie/Pankreas-und-Milz/Pyloruserhaltende-Duodenohemipankreatektomie-nach-Traverso-Longmire-mit-T-Drainage-pp-Whipple">https://www.webop.de/Allgemein-und-Viszeralchirurgie/Pankreas-und-Milz/Pyloruserhaltende-Duodenohemipankreatektomie-nach-Traverso-Longmire-mit-T-Drainage-pp-Whipple</a> (2019)</li><li>Machado, M. C., Machado, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+use+of+isolated+pancreatic+anastomosis+after+pancreatoduodenectomy:+Five+years+of+experience+with+zero+mortality.">Systematic use of isolated pancreatic anastomosis after pancreatoduodenectomy: Five years of experience with zero mortality.</a> European Journal of Surgical Oncology. 42 (10), 1584-1590 (2016).</li><li>Pescio, G., Cariati, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+reconstructive+method+after+pancreaticoduodenectomy:+the+triple+Roux+on+a+"P"+loop.+Rationale+and+radionuclide+scanning+evaluation.">A new reconstructive method after pancreaticoduodenectomy: the triple Roux on a "P" loop. Rationale and radionuclide scanning evaluation.</a> HPB Surgery. 9 (4), 223-227 (1996).</li><li>Kingsnorth, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Safety+and+function+of+isolated+Roux+loop+pancreaticojejunostomy+after+Whipple's+pancreaticoduodenectomy.">Safety and function of isolated Roux loop pancreaticojejunostomy after Whipple's pancreaticoduodenectomy.</a> Annals of the Royal College of Surgeons of England. 76 (3), 175-179 (1994).</li><li>Jester, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Impact+of+Hepaticojejunostomy+Leaks+After+Pancreatoduodenectomy:+a+Devastating+Source+of+Morbidity+and+Mortality.">The Impact of Hepaticojejunostomy Leaks After Pancreatoduodenectomy: a Devastating Source of Morbidity and Mortality.</a> Journal of Gastrointestinal Surgery. 21 (6), 1017-1024 (2017).</li><li>Funovics, J. M., Zoch, G., Wenzl, E., Schulz, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Progress+in+reconstruction+after+resection+of+the+head+of+the+pancreas.">Progress in reconstruction after resection of the head of the pancreas.</a> Surgery Gynecology Obstetrics. 164 (6), 545-548 (1987).</li><li>Jover, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Results+of+defunctionalized+jejunal+loop+after+pancreaticoduodenectomy.">Results of defunctionalized jejunal loop after pancreaticoduodenectomy.</a> Cirurgia Espanola. 80 (6), 373-377 (2006).</li><li>Albertson, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancreaticoduodenectomy+with+reconstruction+by+Roux-en-Y+pancreaticojejunostomy:+no+operative+mortality+in+a+series+of+25+cases.">Pancreaticoduodenectomy with reconstruction by Roux-en-Y pancreaticojejunostomy: no operative mortality in a series of 25 cases.</a> The Southern Medical Journal. 87 (2), 197-201 (1994).</li></ol>

À ce jour, le POPF contribue de manière significative à la morbidité postopératoire après la1.
Un mélange de bile et de jus pancréatique peut conduire à l'activation mutuelle des deux sécrétions, augmentant leurs effets indésirables sur le PJ ou le HJ9,13,14,15,16. L'activité cytotoxique du phosp...

La pancréaticoduodenectomy (PD) est considérée une procédure chirurgicale sophistiquée. Bien que la mortalité ait diminué au cours des dernières décennies, la morbidité demeure élevée1. La complication la plus fréquente et redoutée est la fistule pancréatique postopératoire (POPF), qui se produit dans plus de 20% des opérations2,3,4,5. POPF peut conduire à des complications ultérieures telles que l'hémorragie postpancreatectomie, la vidange gastrique retardée, l'abcès intraabdominal et le septicémie. L'augmentation des coûts hospitaliers, le séjour prolongé à l'hôpital et le retard dans l'initiation de la chimiothérapie chez les patients atteints de cancer ont un impact socio-économique dramatique2,3,4.
Différentes approches chirurgicales ont été introduites au cours des dernières décennies pour diminuer la fréquence et la gravité du POPF. L'un d'eux est la reconstruction à double boucle (DLR) avec des boucles isolées De Roux-en-Y pour les anastomosesbilitiques et pancréatiques. Séparer le jus pancréatique de la bile est pensé pour diminuer les effets néfastes des sécrétions mutuellement activées sur la pancréaticojejunostomy (PJ). Les résultats dans la littérature ont été encourageants6,7,8. Cependant, le temps opératoire est considérablement prolongé avec DLR4,9.
Nous introduisons ici la technique de reconstruction modifiée à boucle unique (mSLR) pendant la pour séparer la sécrétion pancréatique de la bile par rapport à la reconstruction conventionnelle à une boucle (SLR, figure 1). Dans cette méthode, une seule longue boucle de petit-bowel est utilisée pour la construction de l'hépaticojejunostomie (HJ) et PJ. Une anastomose d'un côté à l'autre entre le membre afféentant et efferent du HJ facilite le flux isolé des sécrétions biliaires et pancréatiques. Les premiers résultats prometteurs confirmant la mortalité et la morbidité diminuées dans les patients présentant le pancreata à haut risque ont été rapportés par notre groupe en 201810.
Cette méthode réduit la sévérité de POPF en séparant le fluide pancréatique et la bile sans prolonger la durée de la chirurgie de manière significative par rapport à la reconstruction à double boucle.

<table>
	<tbody>
		<tr>
			<td>Alexis O XXL</td>
			<td>Applied medical</td>
			<td>C8405</td>
			<td></td>
		</tr>
		<tr>
			<td>Bauchtuch Gr&#252;n 10x90</td>
			<td>Nobatrast</td>
			<td>603219</td>
			<td>abdominal compress</td>
		</tr>
		<tr>
			<td>Bauchtuch Gr&#252;n 45x45</td>
			<td>Nobatrast</td>
			<td>607544</td>
			<td>abdominal compress</td>
		</tr>
		<tr>
			<td>GIA 60mm-3.8mm</td>
			<td>Covidien/Medtronic</td>
			<td>GIA6038S</td>
			<td></td>
		</tr>
		<tr>
			<td>GIA Loading Unit</td>
			<td>Covidien/Medtronic</td>
			<td>GIA6038L</td>
			<td></td>
		</tr>
		<tr>
			<td>OP-Flex Yankauer</td>
			<td>Unomedical</td>
			<td>34094182</td>
			<td>suction device</td>
		</tr>
		<tr>
			<td>Pr&#228;pariertupfer-Set</td>
			<td>Fuhrmann</td>
			<td>32019</td>
			<td>sterile swab</td>
		</tr>
		<tr>
			<td>Schlinggazetupfer 30x40</td>
			<td>Fuhrmann</td>
			<td>32016</td>
			<td>sterile swab</td>
		</tr>
		<tr>
			<td>Telescopic Smoke Pencil</td>
			<td>LiNA Medical</td>
			<td>SHK-TSP-CL</td>
			<td>electro cautery</td>
		</tr>
		<tr>
			<td>Thermocover ECO</td>
			<td>PEMAX</td>
			<td>TC43-75EC2</td>
			<td>bowel cover</td>
		</tr>
		<tr>
			<td>Whipple-Set</td>
			<td>M&#246;lnlycke</td>
			<td>97016042-00</td>
			<td></td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Drainages:</td>
		</tr>
		<tr>
			<td>Drainagebeutel</td>
			<td>Coloplast</td>
			<td>?</td>
			<td>drainagebag</td>
		</tr>
		<tr>
			<td>Silikon-Kapillardrainage</td>
			<td>AsidBonz</td>
			<td>551012</td>
			<td>capillary drainage</td>
		</tr>
		<tr>
			<td>Trichteransatz f&#252;r T-Rohr</td>
			<td>R&#252;sch</td>
			<td>333169-000025</td>
			<td>2,5 mm</td>
		</tr>
		<tr>
			<td>T-Rohr nach Kehr</td>
			<td>R&#252;sch</td>
			<td>423600-000025</td>
			<td>t-tube 2,5 mm</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Sutures:</td>
		</tr>
		<tr>
			<td>Ethiloop 2mm 2x45cm</td>
			<td>Ethicon</td>
			<td>EH387</td>
			<td>loop</td>
		</tr>
		<tr>
			<td>Mersilene 0 FSL</td>
			<td>Ethicon</td>
			<td>EH7637</td>
			<td>fixation of the drainage</td>
		</tr>
		<tr>
			<td>PDS 1 CT</td>
			<td>Ethicon</td>
			<td>PDP9234</td>
			<td>suturing of the fascia</td>
		</tr>
		<tr>
			<td>Vicryl 2-0 , 6x45cm</td>
			<td>Ethicon</td>
			<td>VCP1226</td>
			<td>for ligature</td>
		</tr>
		<tr>
			<td>Vicryl 2-0 SHplus, 8x70</td>
			<td>Ethicon</td>
			<td>VCP7850</td>
			<td>for ligature</td>
		</tr>
		<tr>
			<td>Vicryl 3-0 MH-1plus</td>
			<td>Ethicon</td>
			<td>VCP245</td>
			<td>subcutaneous suture</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Pancreatic anastomoses:</td>
		</tr>
		<tr>
			<td>Prolene 5-0 c1</td>
			<td>Ethicon</td>
			<td>8890</td>
			<td>6 x</td>
		</tr>
		<tr>
			<td>PDS 5.0 RB-1plus</td>
			<td>Ethicon</td>
			<td>X1153</td>
			<td>2 x Multipack</td>
		</tr>
		<tr>
			<td>Prolene 4-0 RB-1</td>
			<td>Ethicon</td>
			<td>8871</td>
			<td>2 x holding sutures</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Hepaticojejunostomy:</td>
		</tr>
		<tr>
			<td>Prolene 5-0 c1</td>
			<td>Ethicon</td>
			<td>8890</td>
			<td>2 x holding sutures</td>
		</tr>
		<tr>
			<td>PDS 5-0 c1</td>
			<td>Ethicon</td>
			<td>PDP1013</td>
			<td>7 x</td>
		</tr>
		<tr>
			<td>PDS 6-0 c1</td>
			<td>Ethicon</td>
			<td>PDP1012</td>
			<td>1 x mucosa fixation sutures</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Gastrojejunostomy:</td>
		</tr>
		<tr>
			<td>PDS 4-0 SH-1 plus</td>
			<td>Ethicon</td>
			<td>X1154</td>
			<td>1 x Multipack</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Jejuno-Jejunostomy</td>
		</tr>
		<tr>
			<td>Ligasure</td>
			<td>Covidien</td>
			<td>LS1520</td>
			<td></td>
		</tr>
		<tr>
			<td>PDS 5-0 RB-1 plus</td>
			<td>Ethicon</td>
			<td>X1153</td>
			<td>1 x Multipack</td>
		</tr>
		<tr>
			<td>Prolene 2-0 SH</td>
			<td>Ethicon</td>
			<td>8833</td>
			<td>Vessel-suture, etc.</td>
		</tr>
		<tr>
			<td>Prolene 3-0 SH</td>
			<td>Ethicon</td>
			<td>8832</td>
			<td>Cystic duct, cystic artery</td>
		</tr>
		<tr>
			<td>Prolene 4-0 RB-1</td>
			<td>Ethicon</td>
			<td>8871</td>
			<td>2 mal suturing of the slit in the meso</td>
		</tr>
	</tbody>
</table>

modified single-loop reconstruction for pancreaticoduodenectomy

La reconstruction modifiée de simple boucle dans le pancreaticoduodenectomy sépare la sécrétion pancréatique de la bile. Il est exécuté dans les cas des restes pancréatiques à haut risque pour réduire la sévérité des fistules pancréatiques postopératoires et en outre la morbidité postopératoire globale. Cette technique de reconstruction est caractérisée par une boucle jejunal extra-longue pour la construction de la pancréaticojejunostomy et de l'hépaticojejunostomy. La plus longue distance entre ces anastomoses et un jejunostomy additionnel entre le membre afféentant et efferent de l'hépaticojejunostomy séparent les fluides et empêchent le reflux de la bile vers la pancréaticojejunostomy. Ainsi, les sécrétions ne peuvent pas s'activer les unes les autres et aggraver une fuite anastomotique existante. Nous avons observé un taux réduit des fistules pancréatiques postopératoires graves après reconstruction simple-boucle modifiée comparée à la reconstruction simple conventionnelle de boucle. La technique est facile à exécuter, sûre et moins longue qu'une reconstruction traditionnelle en double boucle.

Chaque patient subissant a subi une recherche du tissu pancréatique pour évaluer l'indication pour mSLR. Des restes pancréatiques à haut risque (c.-à-d., tissu pancréatique mou et un petit conduit pancréatique principal) ont été reconstruits à l'aide d'une boucle unique modifiée chaque fois que possible.
Nous avons identifié 50 patients présentant le tissu pancréatique à haut risque et un petit conduit pancréatique principal. Tous ont subi mSLR. L'âge moyen était de 63 à 13 ...

Watch this Scientific Journal Video about Modified Single-Loop Reconstruction for Pancreaticoduodenectomy at JoVE.com

Modified Single-Loop Reconstruction for Pancreaticoduodenectomy

La technique de reconstruction à boucle simple modifiée suivant pancreaticoduodenectomy sépare la sécrétion pancréatique de la bile, réduisant ainsi la sévérité de POPF sans prolonger la durée de la chirurgie.

Reconstruction à boucle unique modifiée pour pancreaticoduodenectomy

Modified single-loop reconstruction in pancreaticoduodenectomy separates pancreatic secretion from bile. It is performed in cases of high-risk pancreatic ...

La séparation de la sécrétion pancréatique de la bile diminue les effets préjudiciables des sécrétions mutuellement activées sur le pancreaticoduodenostomy. Ceci réduit la sévérité, mais pas l’incidence, de la fistule pancréatique postopératoire. La reconstruction d’une seule boucle est une technique de reconstruction fréquemment exécutée dans pancreaticoduodenectomy.La reconstruction en double boucle a été introduite pour la première fois en 1976. Il se rend compte de la théorie de la séparation du liquide pancréatique et de la bile. Toutefois, cette procédure prend beaucoup plus de temps que la reconstruction classique en boucle unique.La reconstruction modifiée d’une seule boucle vise à séparer le fluide pancréatique de la bile avec un long membre jejunal et une anastomosis jejunal supplémentaire entre le membre afferent et efferent de l’hépaticojejunostomy. Cette image démontre la phase de reconstruction finie d’un pancreaticoduodenectomy avec une reconstruction modifiée d’une seule boucle. Les flèches vertes démontrent la pancréaticojejunostomy, l’hepaticojejunostomy, et la jejunojejunostomy.Le membre jejunal extra long peut être vu clairement dans cette image. Le protocole d’étude de cette étude portant uniquement sur la chirurgie humaine a été approuvé par le Comité d’éthique de la Ruhr-Université de Bochum. Le consentement éclairé écrit a été obtenu de tous les patients.C’est la préparation d’un patch ligamentaire teres. Il sera enroulé autour de l’artère hépatique après l’achèvement de l’anastomose pour réduire le risque de saignement d’érosion en cas de fuites anastomotic. Commencez la phase de reconstruction par l’immobilisation d’une longue boucle jejunal mobile.Resect les 10 premiers pouces du jejunum pour fournir assez de longueur de la mesenterie. Reposit la boucle jejunal dans le quadrant abdominal supérieur droit. Effectuez une pancréaticojejunostomie intersite à double couche.Utilisez des sutures de polydioxanone interrompues 5-0 pour la couche externe et des sutures interrompues de polypropylène 5-0 pour la couche interne. Les enfiles ou les scellants ne sont pas nécessaires. Cette séquence montre la suture de la couche interne de la pancréaticojejunostomy avec une suture interrompue de polypropylène 5-0.Placez les sutures près les unes des autres pour éviter une insuffisance de l’anastomose. Ne placez pas les sutures trop près les unes des autres pour éviter la nécrose du tissu suture. Ici nous voyons la construction de la couche externe de la pancréaticojejunostomy avec une suture interrompue de polydioxanone 5-0.Les sutures sont préparées comme démontré dans cette séquence. Encore une fois, ne placez pas les sutures trop près les unes des autres. Il s’agit de la double couche finie dans le conduit-à-muqueuse pancreaticojejunostomy.Construire le mur extérieur de la jejunojejunostomy avant de construire l’hépaticojejunostomy. Cela facilite la construction de l’hépaticojejunostomy. Maintenant, nous pouvons clairement voir comment le long membre jejunal va être placé.La boucle jejunal entre hepaticojejunostomy et pancreaticojejunostomy mesure 25 à 35 centimètres au lieu de 10 centimètres. Cela crée une distance entre l’anastomose pour le détournement des sécrétions. Il facilite en outre la construction d’une jejunojejunostomy supplémentaire entre le membre afferent et efferent de l’hepaticojejunostomy.Effectuez une suture de polydioxanone interrompue à une couche 5-0 pour l’hépaticojejunostomy. Fendre les canaux biliaires communs à paroi mince et minuscules à l’intérieur d’un tube T. Commencez l’hepaticojejunostomy avec quatre sutures de fixation de muqueuse.Deux sutures de fixation en polypropylène 5-0 facilitent la construction de l’hépaticojejunostomy. Commencez la construction de l’hépaticojejunostomy avec un Roux-en-Y utilisant des sutures interrompues de polydioxanone 5-0. Préparez les nœuds chirurgicaux mais ne les attachez pas au début.Les nœuds chirurgicaux préparés sont attachés par la suite pour permettre une tension égale sur chaque suture. Ici, nous pouvons voir clairement le membre jejunal extra long pour une reconstruction modifiée en boucle unique. Ici, nous voyons la construction de la paroi antérieure de l’hépaticojejunostomy.La jejenojejunostomy est une anastomose jejunal à double couche de 1,5 centimètre réalisée avec une suture polydioxanone 5-0. Construire l’anastomose au point le plus bas entre le membre efferent et afferent de l’hépaticojejunostomy. Construisez cette anastomose de la pancréaticojejunostomy et de l’hépaticojejunostomy parce qu’ils sont les plus sophistiqués.Utilisez la cautérisation monopolisaire pour ouvrir le jejunum pour la construction de jejunojejunostomy. Effectuez une suture continue avec une suture en polydioxanone 5-0 pour la couche intérieure de la paroi arrière de la jejunojejunostomy. La suture continue permet une répartition égale de la tension sur l’anastomose.Ici, nous voyons la construction de la couche interne de la paroi antérieure de l’anastomose. Il s’agit de la construction de la couche extérieure de la paroi antérieure. Ici, nous voyons la pancréaticojejunostomy, la jejunojejunostomy, et l’hepaticojejunostomy à l’arrière-plan.Combler l’écart dans le mésocolon après la transposition du membre jejunal pour éviter l’hernie interne. Chaque patient avec un tissu pancréatique mou et un conduit pancréatique principal minuscule a subi la reconstruction modifiée de single-loop chaque fois que possible. La mobilisation du long membre jejunal n’était habituellement aucun problème.Dans de rares cas, la mobilisation a été limitée en raison de l’obésité viscérale extrême ou des adhérences graves dues aux antécédents chirurgicaux passés. Les petits patients viennent avec l’espace naturellement plus petit dans l’abdomen supérieur, enant la préparation aussi bien. La reconstruction continue de double couche de la jejunojejunostomy a été exécutée sans difficultés dans tous les patients.Aucune insuffisance anastomotic ne s’est produite postopératoirement. Cette méthode réduit l’incidence de fistule pancréatique postopératoire grave dans tous les patients présentant des restes pancréatiques à haut risque. Le nombre d’hémorragies et de réopérations postpancreatectomy était sensiblement inférieur avec la reconstruction modifiée d’une seule boucle.Par rapport à la reconstruction en double boucle, cette procédure est nettement moins exigeante techniquement et moins longue. La reconstruction modifiée d’une seule boucle est une technique simple pour séparer la sécrétion pancréatique de la bile pour la réduction de la morbidité postopératoire. Il est sûr et moins long que la reconstruction en double boucle.

modified-single-loop-reconstruction-for-pancreaticoduodenectomy

Research

JoVE Journal

Medicine

Modified Technique for Coronary Artery Ligation in Mice

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI we need better knowledge about pathophysiology of myocardial ischemia. This knowledge may be valuable to define new therapeutic targets for innovative cardiovascular therapies4. Experimental MI model in mice is an increasingly popular small-animal model in preclinical research in which MI is induced by means of permanent or temporary ligation of left coronary artery (LCA)5. In this video, we describe the step-by-step method of how to induce experimental MI in mice.
The animal is first anesthetized with 2% isoflurane. The unconscious mouse is then intubated and connected to a ventilator for artificial ventilation. The left chest is shaved and 1.5 cm incision along mid-axillary line is made in the skin. The left pectoralis major muscle is bluntly dissociated until the ribs are exposed. The muscle layers are pulled aside and fixed with an eyelid-retractor. After these preparations, left thoracotomy is performed between the third and fourth ribs in order to visualize the anterior surface of the heart and left lung. The proximal segment of LCA artery is then ligated with a 7-0 ethilon suture which typically induces an infarct size ~40% of left ventricle. At the end, the chest is closed and the animals receive postoperative analgesia (Temgesic, 0.3 mg/50 ml, ip). The animals are kept in a warm cage until spontaneous recovery.

The surgical procedure used to induce experimental myocardial infarction in mice begins with left thoracotomy between the third and the fourth ribs in order to visualize the anterior surface of the heart and left lung. The left coronary artery is ligated, the chest is closed and the mouse is allowed to recover spontaneously.

Technique de modification de ligature de l&#39;artère coronaire chez la souris

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

Recherche

Médecine

A Modified Method for Heterotopic Mouse Heart Transplantion

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents available. A difficulty is presented due to the small size of the animal and the considerable technical challenges of the microsurgery involved in heart transplantation. In particular, a high rate of technical failure early after transplantation may result from recipient death and post-operative complications such as hind limb paralysis or a non-beating heart. Here, the complete technique for heterotopic mouse heart transplantation is demonstrated, involving harvesting the donor heart and its subsequent implantation into a recipient mouse. The donor heart is harvested immediately following in situ perfusion with cold heparinized saline and transection of the ascending aorta and pulmonary artery. The recipient operation involves preparation of the abdominal aorta and inferior vena cava (IVC), followed by end-to-side anastomosis of the donor aorta with the recipient aorta using a single running 10-0 microsuture and a similar anastomosis of the donor pulmonary artery with the recipient IVC. Following the operation the animal is injected with 0.6 ml normal saline subcutaneously and allowed to recover on a 37&#160;&#176;C heating pad. The results from 227 mouse heart transplants are summarized with a success rate at 48 hr of 86.8%. Of the 13.2% failures within 48 hr, 5 (2.2%) experienced hind limb paralysis, 10 (4.4%) had a non-beating heart due to graft ischemic injury and/or thrombosis, while 15 (6.6%) died within 48 hr.

A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.

Une méthode modifiée de Heterotopic souris coeur la transplantation

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents ...

Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach

Computer-aided design/computer-assisted manufacturing (CAD/CAM) is now being evaluated as a preparative technique for maxillofacial surgery. Because this technique is expensive and available in only limited areas of the world, we developed a novel CAD/CAM surgical guide using an in-house approach. By using the CAD software, the maxillary resection area and cutting planes and the fibular cutting planes and angles are determined. Once the resection area is decided, the necessary faces are extracted using a Boolean modifier. These superficial faces are united to fit the surface of the bones and thickened to stabilize the solids. Not only the cutting guides for the fibula and maxilla but also the location arrangement of the transferred bone segments is defined by thickening the superficial faces. The CAD design is recorded as .stl files and three-dimensionally (3-D) printed as actual surgical guides. To check the accuracy of the guides, model surgery using 3-D-printed facial and fibular models is performed. These methods may be used to assist surgeons where commercial guides are not available.

Methods for designing a computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guide are shown. Cutting planes are separated, united, and thickened to easily visualize the necessary bone transfer. These designs can be three-dimensional printed and checked for accuracy.

Conception des Guides chirurgicaux CAD/CAM pour Reconstruction maxillaire à l’aide d’une approche interne

Computer-aided design/computer-assisted manufacturing (CAD/CAM) is now being evaluated as a preparative technique for maxillofacial surgery. Because this ...

Porcine As a Training Module for Head and Neck Microvascular Reconstruction

Live models that resemble surgical conditions of humans are needed for training free-flap harvesting and anastomosis. Animal models for training purposes have been available for years in many surgical fields. We used the female (because they are easy to handle for the procedure) Yorkshire pigs for the head and neck reconstruction by harvesting the deep inferior epigastric artery perforator or the superior epigastric artery perforator flap. The anastomosis site (neck skin defect or tracheal wall defect) was prepared via the dissection of the common carotid artery and the internal jugular vein, in which 3.5&#215; loupe magnification was used for anastomosis as we use on human cases in real life. This procedure demonstrates a new training method using a reliable learning model and provides a detailed anatomy in a live scenario. We focused on the ischemia time, harvesting, vessel anastomosis, and designing the flap to fit the defect site. This model improves tissue handling and with the use of proper instruments can be repeated many times so that the surgeon is fully confident before starting the surgery on humans.

Here we present a protocol for the use of the pig superior epigastric artery perforator flap as a learning module for head and neck microvascular reconstruction.

Porcine comme un Module de formation pour la tête et du cou Reconstruction microvasculaire

Live models that resemble surgical conditions of humans are needed for training free-flap harvesting and anastomosis. Animal models for training purposes ...

A Training and Testing System for Performing Vascular Reconstruction In Vitro

Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has yet to be developed. In this study, we introduce an in vitro training and testing system using a magnetic anchoring technique with which a trainee can practice manual vascular reconstruction individually. Additionally, this system can also be used to test the quality of the reconstruction. The described system includes a vascular reconstruction training machine, magnetic tractors, and a magnetic suture puller. In this manuscript, we detail an end-to-end vein anastomosis using porcine right and left iliac veins. To identify the potential damage caused by a magnetic suture puller on the suture, we created three groups with six segments of 4-0 polypropylene sutures each: a control group with no intervention on the polypropylene suture, a group in which the polypropylene suture is manually pulled with sterile gloves 20x, and a magnetic puller group in which the magnetic puller pulled the polypropylene suture 20x. These groups were tested by light microscopy and breaking strength tests, and the effect of reconstruction was assessed. In the light microscopy test, the control group was less likely to be damaged (p &#60; 0.05) and the number of damaged points of the manual group and magnetic puller group were similar (p &#62; 0.05). The results of the breaking strength test were compared across groups and no significant difference was observed (p &#62; 0.05). The end-to-end anastomosis of the porcine iliac veins was successfully performed using this training system, and the reconstructed veins could undergo 2.0 kPa perfusion pressure. Using this training and testing system the trainee can practice manual vascular reconstruction in vitro individually with the aid of magnetic tractors and a magnetic suture puller, and the quality of the reconstruction can be tested.

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

Un système de formation et d'essai pour effectuer la reconstruction vasculaire in Vitro

Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has ...

Technical Detail for Robot Assisted Pancreaticoduodenectomy

Since its first report in 2003, robotic pancreaticoduodenectomy (RPD) has gained popularity among pancreatic surgeons. Inherent advantages of the robotic platform, including three-dimensional vision, wristed instruments, and improved ergonomics, allow the surgeon to recapitulate the principles of open pancreatoduodenectomy allowing safe oncologic dissection, hemostasis, and meticulous reconstruction. Over the course of the past decade, significant strides have been achieved in outlining the safety, feasibility, and learning curve of the robotic Whipple. When performed by high volume pancreatic surgeons experienced in RPD, recent comparative effectiveness studies show potential advantages compared to the open technique, including reductions in hospital stay and morbidity. National data also show reductions in conversion rates compared to its laparoscopic counterpart. Although long-term oncologic data are still needed, short-term oncologic surrogates of margin resection and lymph node harvest suggest no compromise in oncologic outcomes. As pancreatic surgeons increasingly integrate robotics into their practice, proficiency-based training and credentialing will be necessary for the safe application and dissemination of RPD. Here, we provide the detailed steps of a robotic pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.&#160;

The following manuscript details a stepwise approach to the robot-assisted pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.

Détail technique pour robot assisté Pancreaticoduodenectomy

Since its first report in 2003, robotic pancreaticoduodenectomy (RPD) has gained popularity among pancreatic surgeons. Inherent advantages of the robotic ...

A Postoperative Evaluation Guideline for Computer-Assisted Reconstruction of the Mandible

Valid comparisons of postoperative accuracy results in computer-assisted reconstruction of the mandible are difficult due to heterogeneity in imaging modalities, mandibular defect classification, and evaluation methodologies between studies. This guideline uses a step-by-step approach guiding the process of imaging, classification of mandibular defects and volume assessment of three-dimensional (3D) models, after which a legitimized quantitative accuracy evaluation method can be performed between the postoperative clinical situation and the preoperative virtual plan. The condyles and the vertical and horizontal corners of the mandible are used as bony landmarks to define virtual lines in the computer-assisted surgery (CAS) software. Between these lines the axial, coronal, and both sagittal mandibular angles are calculated on both pre- and postoperative 3D models of the (neo)mandible and subsequently the deviations are calculated. By superimposing the postoperative 3D model to the preoperative virtually planned 3D model, which is fixed to the XYZ axis, the deviation between pre- and postoperative virtually planned dental implant positions can be calculated. This protocol continues and specifies an earlier publication of this evaluation guideline.

Here, we propose a practical, feasible and reproducible evaluation guideline for computer-assisted reconstruction of the mandible in order to create uniformity between studies regarding postoperative accuracy evaluation. This protocol continues and specifies an earlier publication of this evaluation guideline.

Une ligne directrice d'évaluation postopératoire pour la reconstruction assistée par ordinateur de la mandibule

Valid comparisons of postoperative accuracy results in computer-assisted reconstruction of the mandible are difficult due to heterogeneity in imaging ...

Therapy Interventions for Upper Limb Amputees Undergoing Selective Nerve Transfers

Targeted Muscle Reinnervation (TMR) improves the biological control interface for myoelectric prostheses after above-elbow amputation. Selective activation of muscle units is made possible by surgically re-routing nerves, yielding a high number of independent myoelectric control signals. However, this intervention requires careful patient selection and specific rehabilitation therapy. Here a rehabilitation protocol is presented for high-level upper limb amputees undergoing TMR, based on an expert Delphi study. Interventions before surgery include detailed patient assessment and general measures for pain control, muscle endurance and strength, balance, and range of motion of the remaining joints. After surgery, additional therapeutic interventions focus on edema control and scar treatment and the selective activation of cortical areas responsible for upper limb control. Following successful&#160;reinnervation of target muscles, surface electromyographic (sEMG) biofeedback is used to train the activation of the novel muscular units. Later on, a table-top prosthesis may provide the first experience of prosthetic control. After fitting the actual prosthesis, training includes repetitive drills without objects, object manipulation, and finally, activities of daily living. Ultimately, regular patient appointments and functional assessments allow tracking prosthetic function and enabling early interventions if malfunctioning.

This work presents a protocol to enhance prosthetic function after selective nerve transfer surgery. Rehabilitation interventions include patient information and selection, support of wound healing, cortical re-activation of sensory-motor areas of the upper limb, training of selective muscle activation, prosthetic handling in daily life, and regular follow-up assessments.

Interventions thérapeutiques pour les amputés des membres supérieurs subissant des transferts nerveux sélectifs

Targeted Muscle Reinnervation (TMR) improves the biological control interface for myoelectric prostheses after above-elbow amputation. Selective ...

Management of the Uncinate Process in No-Touch Laparoscopic Pancreaticoduodenectomy

Laparoscopic pancreatoduodenectomy (LPD) is a demanding abdominal operation that necessitates meticulous surgical skills and teamwork. The management of the pancreatic uncinate process is one of the most important and difficult processes in LPD because of its deep anatomical location and difficult exposure. Complete resection of the uncinate process and mesopancreas has become the cornerstone of LPD. In particular, it is even more difficult to avoid positive surgical margins and incomplete lymph node dissection when the tumor is located in the uncinate process. No-touch LPD, which is an ideal oncological operation process fitting the "tumor-free" principle, has been reported by our group previously. This article introduces the management of the uncinate process in no-touch LPD. Based on the multi-angle arterial approach, in this protocol, the median-anterior and left-posterior approaches to the SMA are used to correctly deal with the important vascular structure, the inferior pancreaticoduodenal artery (IPDA), in order to ensure the safe and complete excision of the uncinate process and mesopancreas. For the achievement of the no-touch isolation technique in LPD, the pancreatic head and the blood supply to the duodenal region must be severed at the very early stage of the operation; after that, the tumor can be isolated intact, resection can be performed in situ, and finally, the tissue can be removed en bloc. This paper aims to show the distinctive ways to manage the uncinate process in no-touch LPD and investigate the viability and safety of this approach. Moreover, the technique may increase the R0 resection rate.

Complete resection of the uncinate process and mesopancreas is one of the most important and difficult processes in laparoscopic pancreatoduodenectomy (LPD). This article presents a method for managing the uncinate process in no-touch LPD using the median-anterior and left-posterior approaches to the superior mesenteric artery (SMA).

Prise en charge de l’apophyse uncinée dans la pancréaticoduodénectomie laparoscopique sans contact

Laparoscopic pancreatoduodenectomy (LPD) is a demanding abdominal operation that necessitates meticulous surgical skills and teamwork. The management of ...

Digital Model for Horizontal 3D Reconstruction of Pulmonary Nodules

To reconstruct the three-dimensional digital model of the pulmonary nodules, implement the Three-Dimensional Lung Horizon Function in the MATLAB workspace. Then open the Graphical User Interface, or GUI, to check the horizontal three-dimensional model. Drag the scroll bar on the GUI with the mouse to observe the continuous three-dimensional lung structure, allowing for the clear visualization of various types of pulmonary nodules and their relative spatial relationships with the lung tissue.Use the Zoom function to observe the local features of the lesions and relevant three-dimensional structural output pictures. To measure the size of the nodules, use the Mark Pixel Coordinates button to calculate the distance between two points. The default color bar, jet colormap, displays values from low to high in a blue to red range.To reset the GUI, right click the color bar and select the standard gray colormap. If the filter window is not satisfactory, use the left mouse button to adjust the window level by dragging up and down in the middle of the figure. Drag left and right to adjust the window width to display the accurate filtering range on the color bar.The continuous three-dimensional reconstruction resulted in the axial view of the pulmonary nodules and the relationship between the nodules and the surrounding lung tissue.