Name: modified single-loop reconstruction for pancreaticoduodenectomy
Uploaded: 2019-09-28T12:30:00
Description: Watch this Scientific Journal Video about Modified Single-Loop Reconstruction for Pancreaticoduodenectomy at JoVE.com

해당되지 않습니다.

이 프로토콜은 mSLR을 사용하여 PD의 재건 단계에 초점을 맞추고 루르 대학 보훔, 독일의 윤리위원회의 지침을 따릅니다.
참고: 췌장두, 유두 부위 또는 심한 난치성 만성 석회화 췌장염을 앓고 있는 환자는 종양이 50% 미만의 경우 췌장동맥에 접촉하지 않았으며 그렇지 않았습니다. 포털 정맥 또는 우수한 간부 정맥에 침투. 선암의 경우에 먼 전이는 또한 PD를 위한 금기이었습니...

<ol>
	<li>Cameron, J. L., He, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+thousand+consecutive+pancreaticoduodenectomies.">Two thousand consecutive pancreaticoduodenectomies.</a> Journal of American College of Surgeons. 220 (4), 530-536 (2015).</li><li>Keck, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancreatogastrostomy+Versus+Pancreatojejunostomy+for+RECOnstruction+After+PANCreatoduodenectomy+(RECOPANC,+DRKS+00000767):+Perioperative+and+Long-term+Results+of+a+Multicenter+Randomized+Controlled+Trial.">Pancreatogastrostomy Versus Pancreatojejunostomy for RECOnstruction After PANCreatoduodenectomy (RECOPANC, DRKS 00000767): Perioperative and Long-term Results of a Multicenter Randomized Controlled Trial.</a> Annals of Surgery. 263 (3), 440-449 (2016).</li><li>Deng, L. H., Xiong, J. J., Xia, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolated+Roux-en-Y+pancreaticojejunostomy+versus+conventional+pancreaticojejunostomy+after+pancreaticoduodenectomy:+a+systematic+review+and+meta-analysis.">Isolated Roux-en-Y pancreaticojejunostomy versus conventional pancreaticojejunostomy after pancreaticoduodenectomy: a systematic review and meta-analysis.</a> Journal of Evidence Based Medicine. 10 (1), 37-45 (2017).</li><li>Klaiber, U., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Meta-analysis+of+complication+rates+for+single-loop+versus+dual-loop+(Roux-en-Y)+with+isolated+pancreaticojejunostomy+reconstruction+after+pancreaticoduodenectomy.">Meta-analysis of complication rates for single-loop versus dual-loop (Roux-en-Y) with isolated pancreaticojejunostomy reconstruction after pancreaticoduodenectomy.</a> British Journal of Surgery. 102 (4), 331-340 (2015).</li><li>Eshmuminov, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+review+and+meta-analysis+of+postoperative+pancreatic+fistula+rates+using+the+updated+2016+International+Study+Group+Pancreatic+Fistula+definition+in+patients+undergoing+pancreatic+resection+with+soft+and+hard+pancreatic+texture.">Systematic review and meta-analysis of postoperative pancreatic fistula rates using the updated 2016 International Study Group Pancreatic Fistula definition in patients undergoing pancreatic resection with soft and hard pancreatic texture.</a> HPB (Oxford). 20 (11), 992-1003 (2018).</li><li>Ke, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+prospective,+randomized+trial+of+Roux-en-Y+reconstruction+with+isolated+pancreatic+drainage+versus+conventional+loop+reconstruction+after+pancreaticoduodenectomy.">A prospective, randomized trial of Roux-en-Y reconstruction with isolated pancreatic drainage versus conventional loop reconstruction after pancreaticoduodenectomy.</a> Surgery. 153 (6), 743-752 (2013).</li><li>El Nakeeb, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolated+Roux+loop+pancreaticojejunostomy+versus+pancreaticogastrostomy+after+pancreaticoduodenectomy:+a+prospective+randomized+study.">Isolated Roux loop pancreaticojejunostomy versus pancreaticogastrostomy after pancreaticoduodenectomy: a prospective randomized study.</a> HPB (Oxford). 16 (8), 713-722 (2014).</li><li>Tani, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Randomized+clinical+trial+of+isolated+Roux-en-Y+versus+conventional+reconstruction+after+pancreaticoduodenectomy.">Randomized clinical trial of isolated Roux-en-Y versus conventional reconstruction after pancreaticoduodenectomy.</a> British Journal of Surgery. 101 (9), 1084-1091 (2014).</li><li>Machado, M. C., da Cunha, J. E., Bacchella, T., Bove, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+technique+for+the+reconstruction+of+the+alimentary+tract+after+pancreatoduodenectomy.">A modified technique for the reconstruction of the alimentary tract after pancreatoduodenectomy.</a> Surgery Gynecology Obstetrics. 143 (2), 271-272 (1976).</li><li>Aghalarov, I., Herzog, T., Uhl, W., Belyaev, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+single-loop+reconstruction+after+pancreaticoduodenectomy+reduces+severity+of+postoperative+pancreatic+fistula+in+high-risk+patients.">A modified single-loop reconstruction after pancreaticoduodenectomy reduces severity of postoperative pancreatic fistula in high-risk patients.</a> HPB (Oxford). 20 (7), 676-683 (2018).</li><li>Marangoni, G., Morris-Stiff, G., Deshmukh, S., Hakeem, A., Smith, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modern+approach+to+teaching+pancreatic+surgery:+stepwise+pancreatoduodenectomy+for+trainees.">A modern approach to teaching pancreatic surgery: stepwise pancreatoduodenectomy for trainees.</a> Journal of Gastrointestinal Surgery. 16 (8), 1597-1604 (2012).</li><li>. Pyloruserhaltende Duodenohemipankreatektomie nach Traverso-Longmire mit T-Drainage (pp-Whipple) Available from: <a target="_blank" href="https://www.webop.de/Allgemein-und-Viszeralchirurgie/Pankreas-und-Milz/Pyloruserhaltende-Duodenohemipankreatektomie-nach-Traverso-Longmire-mit-T-Drainage-pp-Whipple">https://www.webop.de/Allgemein-und-Viszeralchirurgie/Pankreas-und-Milz/Pyloruserhaltende-Duodenohemipankreatektomie-nach-Traverso-Longmire-mit-T-Drainage-pp-Whipple</a> (2019)</li><li>Machado, M. C., Machado, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Systematic+use+of+isolated+pancreatic+anastomosis+after+pancreatoduodenectomy:+Five+years+of+experience+with+zero+mortality.">Systematic use of isolated pancreatic anastomosis after pancreatoduodenectomy: Five years of experience with zero mortality.</a> European Journal of Surgical Oncology. 42 (10), 1584-1590 (2016).</li><li>Pescio, G., Cariati, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+reconstructive+method+after+pancreaticoduodenectomy:+the+triple+Roux+on+a+"P"+loop.+Rationale+and+radionuclide+scanning+evaluation.">A new reconstructive method after pancreaticoduodenectomy: the triple Roux on a "P" loop. Rationale and radionuclide scanning evaluation.</a> HPB Surgery. 9 (4), 223-227 (1996).</li><li>Kingsnorth, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Safety+and+function+of+isolated+Roux+loop+pancreaticojejunostomy+after+Whipple's+pancreaticoduodenectomy.">Safety and function of isolated Roux loop pancreaticojejunostomy after Whipple's pancreaticoduodenectomy.</a> Annals of the Royal College of Surgeons of England. 76 (3), 175-179 (1994).</li><li>Jester, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Impact+of+Hepaticojejunostomy+Leaks+After+Pancreatoduodenectomy:+a+Devastating+Source+of+Morbidity+and+Mortality.">The Impact of Hepaticojejunostomy Leaks After Pancreatoduodenectomy: a Devastating Source of Morbidity and Mortality.</a> Journal of Gastrointestinal Surgery. 21 (6), 1017-1024 (2017).</li><li>Funovics, J. M., Zoch, G., Wenzl, E., Schulz, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Progress+in+reconstruction+after+resection+of+the+head+of+the+pancreas.">Progress in reconstruction after resection of the head of the pancreas.</a> Surgery Gynecology Obstetrics. 164 (6), 545-548 (1987).</li><li>Jover, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Results+of+defunctionalized+jejunal+loop+after+pancreaticoduodenectomy.">Results of defunctionalized jejunal loop after pancreaticoduodenectomy.</a> Cirurgia Espanola. 80 (6), 373-377 (2006).</li><li>Albertson, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancreaticoduodenectomy+with+reconstruction+by+Roux-en-Y+pancreaticojejunostomy:+no+operative+mortality+in+a+series+of+25+cases.">Pancreaticoduodenectomy with reconstruction by Roux-en-Y pancreaticojejunostomy: no operative mortality in a series of 25 cases.</a> The Southern Medical Journal. 87 (2), 197-201 (1994).</li></ol>

현재까지 POPF는 PD1에이어 수술 후 이환율에 크게 기여한다.
담즙과 췌장 주스의 혼합물은 PJ 또는 HJ9,13,14,15,16에대한 부작용을 증가시켜 두 분비물의 상호 활성화로 이어질 수 있습니다. 활성 인산리파제 A2의 세포 독성 활성, 이는 해로?...

췌장 두이코데네절제술 (PD)은 정교한 외과 적 수술로 간주됩니다. 사망률은 지난 수십 년 동안 감소했지만, 사망률은 여전히 높은남아 1. 가장 빈번하고 두려운 합병증은 수술 후 췌장 누공 (POPF)으로 수술2,3,4,5의20 % 이상에서 발생합니다. POPF는 포스트 판과 같은 후속 합병증으로 이어질 수 있습니다createctomy 출혈, 지연 위 비우기, 복부 농양과 패혈증. 증가된 병원비용, 장기간 입원, 암 환자의 화학요법 개시 지연은 극적인 사회경제적 영향을 미친다2,3,4.
POPF의 빈도와 심각도를 감소시키기 위하여 다른 외과 접근은 지난 십년간에서 소개되었습니다. 그 중 하나는 담즙 과 췌장 해부학에 대한 고립 된 Roux-en-Y 루프가있는 이중 루프 재건 (DLR)입니다. 담즙에서 췌장 주스를 분리하는 것은 췌장 제주 절제술 (PJ)에 상호 활성화 분비물의 해로운 영향을 감소시키기 위하여 생각됩니다. 문헌의 결과는6,7,8을장려하고 있다. 그러나 DLR4,9로작동 시간이 크게 연장됩니다.
여기서 는 종래의 단일 루프 재구성에 비해 담즙으로부터 췌장 분비를 분리하는 PD 동안 변형된 단일 루프 재구성(mSLR) 기술을 소개한다(SLR, 도 1). 이 방법에서는, 단일 긴 작은 창자 루프는 간염 제자절제술 (HJ) 및 PJ의 건설을 위해 이용됩니다. HJ의 원심성 및 원심성 사지 사이의 좌우 해부학은 담즙과 췌장 분비물의 분리 된 흐름을 촉진합니다. 고위험췌장 환자의 사망률 감소 및 사망률을 확인하는 첫 번째 유망한 결과는 2018년10월에우리 그룹에 의해 보고되었다.
이 방법은 이중 루프 재건에 비해 수술 기간을 크게 연장하지 않고 췌장액과 담즙을 분리하여 POPF의 중증도를 감소시킨다.

<table>
	<tbody>
		<tr>
			<td>Alexis O XXL</td>
			<td>Applied medical</td>
			<td>C8405</td>
			<td></td>
		</tr>
		<tr>
			<td>Bauchtuch Gr&#252;n 10x90</td>
			<td>Nobatrast</td>
			<td>603219</td>
			<td>abdominal compress</td>
		</tr>
		<tr>
			<td>Bauchtuch Gr&#252;n 45x45</td>
			<td>Nobatrast</td>
			<td>607544</td>
			<td>abdominal compress</td>
		</tr>
		<tr>
			<td>GIA 60mm-3.8mm</td>
			<td>Covidien/Medtronic</td>
			<td>GIA6038S</td>
			<td></td>
		</tr>
		<tr>
			<td>GIA Loading Unit</td>
			<td>Covidien/Medtronic</td>
			<td>GIA6038L</td>
			<td></td>
		</tr>
		<tr>
			<td>OP-Flex Yankauer</td>
			<td>Unomedical</td>
			<td>34094182</td>
			<td>suction device</td>
		</tr>
		<tr>
			<td>Pr&#228;pariertupfer-Set</td>
			<td>Fuhrmann</td>
			<td>32019</td>
			<td>sterile swab</td>
		</tr>
		<tr>
			<td>Schlinggazetupfer 30x40</td>
			<td>Fuhrmann</td>
			<td>32016</td>
			<td>sterile swab</td>
		</tr>
		<tr>
			<td>Telescopic Smoke Pencil</td>
			<td>LiNA Medical</td>
			<td>SHK-TSP-CL</td>
			<td>electro cautery</td>
		</tr>
		<tr>
			<td>Thermocover ECO</td>
			<td>PEMAX</td>
			<td>TC43-75EC2</td>
			<td>bowel cover</td>
		</tr>
		<tr>
			<td>Whipple-Set</td>
			<td>M&#246;lnlycke</td>
			<td>97016042-00</td>
			<td></td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Drainages:</td>
		</tr>
		<tr>
			<td>Drainagebeutel</td>
			<td>Coloplast</td>
			<td>?</td>
			<td>drainagebag</td>
		</tr>
		<tr>
			<td>Silikon-Kapillardrainage</td>
			<td>AsidBonz</td>
			<td>551012</td>
			<td>capillary drainage</td>
		</tr>
		<tr>
			<td>Trichteransatz f&#252;r T-Rohr</td>
			<td>R&#252;sch</td>
			<td>333169-000025</td>
			<td>2,5 mm</td>
		</tr>
		<tr>
			<td>T-Rohr nach Kehr</td>
			<td>R&#252;sch</td>
			<td>423600-000025</td>
			<td>t-tube 2,5 mm</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Sutures:</td>
		</tr>
		<tr>
			<td>Ethiloop 2mm 2x45cm</td>
			<td>Ethicon</td>
			<td>EH387</td>
			<td>loop</td>
		</tr>
		<tr>
			<td>Mersilene 0 FSL</td>
			<td>Ethicon</td>
			<td>EH7637</td>
			<td>fixation of the drainage</td>
		</tr>
		<tr>
			<td>PDS 1 CT</td>
			<td>Ethicon</td>
			<td>PDP9234</td>
			<td>suturing of the fascia</td>
		</tr>
		<tr>
			<td>Vicryl 2-0 , 6x45cm</td>
			<td>Ethicon</td>
			<td>VCP1226</td>
			<td>for ligature</td>
		</tr>
		<tr>
			<td>Vicryl 2-0 SHplus, 8x70</td>
			<td>Ethicon</td>
			<td>VCP7850</td>
			<td>for ligature</td>
		</tr>
		<tr>
			<td>Vicryl 3-0 MH-1plus</td>
			<td>Ethicon</td>
			<td>VCP245</td>
			<td>subcutaneous suture</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Pancreatic anastomoses:</td>
		</tr>
		<tr>
			<td>Prolene 5-0 c1</td>
			<td>Ethicon</td>
			<td>8890</td>
			<td>6 x</td>
		</tr>
		<tr>
			<td>PDS 5.0 RB-1plus</td>
			<td>Ethicon</td>
			<td>X1153</td>
			<td>2 x Multipack</td>
		</tr>
		<tr>
			<td>Prolene 4-0 RB-1</td>
			<td>Ethicon</td>
			<td>8871</td>
			<td>2 x holding sutures</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Hepaticojejunostomy:</td>
		</tr>
		<tr>
			<td>Prolene 5-0 c1</td>
			<td>Ethicon</td>
			<td>8890</td>
			<td>2 x holding sutures</td>
		</tr>
		<tr>
			<td>PDS 5-0 c1</td>
			<td>Ethicon</td>
			<td>PDP1013</td>
			<td>7 x</td>
		</tr>
		<tr>
			<td>PDS 6-0 c1</td>
			<td>Ethicon</td>
			<td>PDP1012</td>
			<td>1 x mucosa fixation sutures</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Gastrojejunostomy:</td>
		</tr>
		<tr>
			<td>PDS 4-0 SH-1 plus</td>
			<td>Ethicon</td>
			<td>X1154</td>
			<td>1 x Multipack</td>
		</tr>
		<tr>
			<td colspan="4"></td>
		</tr>
		<tr>
			<td colspan="4">Jejuno-Jejunostomy</td>
		</tr>
		<tr>
			<td>Ligasure</td>
			<td>Covidien</td>
			<td>LS1520</td>
			<td></td>
		</tr>
		<tr>
			<td>PDS 5-0 RB-1 plus</td>
			<td>Ethicon</td>
			<td>X1153</td>
			<td>1 x Multipack</td>
		</tr>
		<tr>
			<td>Prolene 2-0 SH</td>
			<td>Ethicon</td>
			<td>8833</td>
			<td>Vessel-suture, etc.</td>
		</tr>
		<tr>
			<td>Prolene 3-0 SH</td>
			<td>Ethicon</td>
			<td>8832</td>
			<td>Cystic duct, cystic artery</td>
		</tr>
		<tr>
			<td>Prolene 4-0 RB-1</td>
			<td>Ethicon</td>
			<td>8871</td>
			<td>2 mal suturing of the slit in the meso</td>
		</tr>
	</tbody>
</table>

modified single-loop reconstruction for pancreaticoduodenectomy

췌장 염기양 절제술에서 수정 된 단일 루프 재건은 담즙에서 췌장 분비를 분리합니다. 그것은 수술 후 췌장 누공의 심각성을 감소시키기 위해 고위험 췌장 잔해의 경우에 수행되고 또한 전반적인 수술 후 이환율. 이러한 재건 기술은 췌장제주절제술과 간염제분과절제술의 시공을 위한 초장거리 제자루프를 특징으로 한다. 이 해부학과 간염의 원심성 및 원심성 사지 사이 추가 jejunostomy 사이 더 긴 거리는 액체를 분리하고 췌장 제주 절제술을 향해 담즙의 역류를 방지합니다. 따라서 분비물은 서로를 활성화시키고 기존의 해부학 적 누출을 악화시킬 수 없습니다. 우리는 기존의 단일 루프 재건에 비해 수정 된 단일 루프 재건 후 심각한 수술 후 췌장 누공의 감소 속도를 관찰했다. 이 기술은 기존의 이중 루프 재구성보다 수행하기 쉽고 안전하며 시간이 덜 걸립니다.

PD를 겪고 있는 모든 환자는 mSLR에 대한 표시를 평가하기 위하여 췌장 조직의 조사를 겪었습니다. 고위험 췌장 잔해 (즉, 연약한 췌장 조직 및 작은 주요 췌장 덕트)는 가능한 한 수정된 단 하나 루프를 사용하여 재건되었습니다.
우리는 고위험 췌장 조직 및 작은 주요 췌장 덕트를 가진 50명의 환자를 확인했습니다. 그들 모두는 mSLR을 시행했다. 평균 연령은 63±13세였다. 남성/여...

Watch this Scientific Journal Video about Modified Single-Loop Reconstruction for Pancreaticoduodenectomy at JoVE.com

Modified Single-Loop Reconstruction for Pancreaticoduodenectomy

췌장 편도체 절제술 다음 수정 된 단일 루프 재건 기술은 담즙에서 췌장 분비를 분리하여 수술 기간을 연장하지 않고 POPF의 심각성을 감소시킵니다.

췌장 편도체 절제술을 위한 수정된 단하나 루프 재건

Modified single-loop reconstruction in pancreaticoduodenectomy separates pancreatic secretion from bile. It is performed in cases of high-risk pancreatic ...

담즙에서 췌장 분비물의 분리는 췌장코듀오데노토미에 상호 활성화된 분비물의 해로운 영향을 감소시다. 이것은 수술 후 췌장 누공의 심각도, 그러나 부각을 감소시킵니다. 단일 루프 재구성은 췌장십절제술에서 자주 수행되는 재구성 기술입니다.이중 루프 재건은 1976년에 처음 도입되었습니다. 그것은 췌장 유체와 담즙의 분리 이론을 실현한다. 그러나, 이 절차는 기존의 단일 루프 재구성보다 훨씬 더 많은 시간이 소요된다.수정된 단일 루프 재건은 긴 제주 사지와 간장 절제술의 발신과 efferent 사지 사이 추가 제주 해부학으로 담즙에서 췌장액을 분리하는 것을 목표로합니다. 이 그림은 수정 된 단일 루프 재구성과 췌장 두각 해독제의 완성 된 재건 단계를 보여줍니다. 녹색 화살은 췌장제주절제술, 간제주절제술, 제주노제노절제술을 보여줍니다.이 그림에서는 여분의 긴 제주 사지를 명확하게 볼 수 있습니다. 인간 수술을 관련시키는 이 연구 결과의 연구 프로토콜은 보훔의 Ruhr-대학에 윤리위원회에 의해서만 승인되었습니다. 서면 통보 된 동의는 모든 환자로부터 얻어졌다.이것은 테레스 인대 패치의 준비입니다. 그것은 해부학 누출의 경우에 침식 출혈의 리스크를 감소시키기 위하여 해부학의 완료 후에 간 동맥을 감싸는 것입니다. 길고 이동식 제주 루프의 고정으로 재건 단계를 시작합니다.제주의 첫 10인치를 절제하여 충분한 길이의 장간을 제공합니다. 오른쪽 상부 복부 사분면에 제주 루프를 리포지트합니다. 이중 층 간 덕트 - 점막 췌장제주 절제술을 수행합니다.외부 층에 대한 중단 된 폴리디옥사넨 5-0 봉합사를 사용하고 내부 층을위한 폴리 프로필렌 5-0 봉합사를 중단하십시오. 스텐트 또는 실란트는 필요하지 않습니다. 이 서열은 중단된 폴리프로필렌 5-0 봉합사를 가진 췌장제주노절제술의 내부 층의 봉합사를 나타낸다.편합사를 서로 가까이 두어 해부학의 부족함을 피하십시오. 봉합사의 괴사를 피하기 위해 봉합사를 서로 너무 가까이 두지 마십시오. 여기서 우리는 중단 된 폴리 디옥사네 5-0 봉합사와 췌장 제주절제술의 외부 층의 건설을 참조하십시오.봉합사는 이 순서에서 입증된 대로 준비됩니다. 다시 한번 봉합사를 서로 너무 가깝게 두지 마십시오. 이것은 덕트 - 점막 췌장내 절제술에 완성 된 이중 층입니다.제주도절제술을 하기 전에 제주노제절제술의 외벽을 건설한다. 이것은 간 절제술의 건설을 용이하게합니다. 이제 우리는 제주 사지가 얼마나 오래 배치될 것인지 를 분명히 알 수 있습니다.제주항과 췌장절제술 사이의 제주고리는 10cm가 아닌 25~35cm를 측정한다. 이렇게 하면 분비물의 전환을 위해 해부학 사이의 거리가 생성됩니다. 또한 간절제 절제술의 발신과 효능 사지 사이에 추가 적인 제주노노절제술의 건설을 용이하게 한다.간 절제절제술을 위한 1층 중단 폴리디옥사네 5-0 봉합사를 수행한다. T 튜브와 얇은 벽과 작은 일반적인 담관을 분할. 4개의 점막 고정 봉합사로 간절제술을 시작합니다.2개의 폴리프로필렌 5-0 보유 봉합사는 간절제 절제술의 건설을 용이하게 합니다. 중단된 폴리디옥사넨 5-0 봉합사를 사용하여 루앙Y로 간절절제술을 시작한다. 수술 매듭을 준비하지만 처음에 그들을 묶어하지 않습니다.준비된 수술 매듭은 각 봉합사에 동등한 긴장을 가능하게 하기 위하여 그 후에 묶여 있습니다. 여기서 우리는 수정 된 단일 루프 재건을위한 여분의 긴 제주 사지를 명확하게 볼 수 있습니다. 여기서 우리는 간 수제 절제술의 전방 벽의 건설을 참조하십시오.제노제주절제술은 폴리디옥사노네 5-0 봉합사로 수행된 2층 제주해부학을 측정하는 1.5센티미터이다. 간 절제절제술의 효능과 발신 사지 사이의 가장 낮은 지점에서 해부학을 구성한다. 췌장제주절제술과 간절제술의 이 해부학을 가장 정교하기 때문에 구성한다.단극성 소어를 사용하여 제주노제노노절제술을 위해 제주를 엽니다. 제주노제노절제술의 후면벽 의 내부층을 위해 폴리디옥사네 5-0 봉합사를 연속봉합사로 수행한다. 지속적인 봉합은 해부학에 긴장의 동등한 분포를 허용합니다.여기서 우리는 해부학의 전방 벽의 내부 층의 구조를 참조하십시오. 이것은 전방 벽의 외부 층의 구조입니다. 여기서 췌장내절제술, 제주노제절제술, 그리고 백그라운드에서 간제주절제술을 볼 수 있습니다.내탈론을 피하기 위해 제주 사지의 전치 후 메소콜론의 간격을 닫는다. 연약한 췌장 조직과 작은 주요 췌장 덕트를 가진 모든 환자는 가능하면 변형 된 단일 루프 재건을 받았다. 긴 제주 사지의 동원은 보통 아무런 문제가 없었다.드문 경우에, 동원은 과거의 외과 역사 때문에 극단적인 내장 비만 또는 가혹한 유착 때문에 제한되었습니다. 작은 환자는 상부 복부에 있는 자연적으로 더 작은 공간과 더불어, 또한 준비를 방해합니다. 제주노제노절제술의 연속 이중층 재건은 모든 환자에서 어려움 없이 수행되었다.마취 부전은 수술 후 발생하지 않았습니다. 이 방법은 고위험 췌장 잔재를 가진 모든 환자에 있는 가혹한 수술 후 췌장 누공의 부각을 감소시킵니다. 변형된 단일 루프 재구성으로 후공 절제술 출혈 및 재작업의 수는 현저히 낮았습니다.이중 루프 재구성과 비교하여 이 절차는 기술적으로 훨씬 덜 까다롭고 시간이 많이 소요됩니다. 수정된 단일 루프 재구성은 수술 후 이환율의 감소를 위해 담즙에서 췌장 분비를 분리하는 간단한 기술입니다. 이중 루프 재구성보다 안전하고 시간이 많이 소요됩니다.

modified-single-loop-reconstruction-for-pancreaticoduodenectomy

Research

JoVE Journal

Medicine

Modified Technique for Coronary Artery Ligation in Mice

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI we need better knowledge about pathophysiology of myocardial ischemia. This knowledge may be valuable to define new therapeutic targets for innovative cardiovascular therapies4. Experimental MI model in mice is an increasingly popular small-animal model in preclinical research in which MI is induced by means of permanent or temporary ligation of left coronary artery (LCA)5. In this video, we describe the step-by-step method of how to induce experimental MI in mice.
The animal is first anesthetized with 2% isoflurane. The unconscious mouse is then intubated and connected to a ventilator for artificial ventilation. The left chest is shaved and 1.5 cm incision along mid-axillary line is made in the skin. The left pectoralis major muscle is bluntly dissociated until the ribs are exposed. The muscle layers are pulled aside and fixed with an eyelid-retractor. After these preparations, left thoracotomy is performed between the third and fourth ribs in order to visualize the anterior surface of the heart and left lung. The proximal segment of LCA artery is then ligated with a 7-0 ethilon suture which typically induces an infarct size ~40% of left ventricle. At the end, the chest is closed and the animals receive postoperative analgesia (Temgesic, 0.3 mg/50 ml, ip). The animals are kept in a warm cage until spontaneous recovery.

The surgical procedure used to induce experimental myocardial infarction in mice begins with left thoracotomy between the third and the fourth ribs in order to visualize the anterior surface of the heart and left lung. The left coronary artery is ligated, the chest is closed and the mouse is allowed to recover spontaneously.

마우스의 관상 동맥 결합에 대한 수정 기술

Myocardial infarction (MI) is one of the most important causes of mortality in humans1-3. In order to improve morbidity and mortality in patients with MI ...

연구

의학

A Modified Method for Heterotopic Mouse Heart Transplantion

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents available. A difficulty is presented due to the small size of the animal and the considerable technical challenges of the microsurgery involved in heart transplantation. In particular, a high rate of technical failure early after transplantation may result from recipient death and post-operative complications such as hind limb paralysis or a non-beating heart. Here, the complete technique for heterotopic mouse heart transplantation is demonstrated, involving harvesting the donor heart and its subsequent implantation into a recipient mouse. The donor heart is harvested immediately following in situ perfusion with cold heparinized saline and transection of the ascending aorta and pulmonary artery. The recipient operation involves preparation of the abdominal aorta and inferior vena cava (IVC), followed by end-to-side anastomosis of the donor aorta with the recipient aorta using a single running 10-0 microsuture and a similar anastomosis of the donor pulmonary artery with the recipient IVC. Following the operation the animal is injected with 0.6 ml normal saline subcutaneously and allowed to recover on a 37&#160;&#176;C heating pad. The results from 227 mouse heart transplants are summarized with a success rate at 48 hr of 86.8%. Of the 13.2% failures within 48 hr, 5 (2.2%) experienced hind limb paralysis, 10 (4.4%) had a non-beating heart due to graft ischemic injury and/or thrombosis, while 15 (6.6%) died within 48 hr.

A technique is demonstrated for the microsurgical procedure for heterotopic transplantation of hearts in mice, including simplified methods for donor harvesting and recipient vessel anastomosis.

이소성 마우스 심장 장기 이식에 대한 수정 방법

Mice are often used as heart transplant donors and recipients in studies of transplant immunology due to the wide range of transgenic mice and reagents ...

Designing CAD/CAM Surgical Guides for Maxillary Reconstruction Using an In-house Approach

Computer-aided design/computer-assisted manufacturing (CAD/CAM) is now being evaluated as a preparative technique for maxillofacial surgery. Because this technique is expensive and available in only limited areas of the world, we developed a novel CAD/CAM surgical guide using an in-house approach. By using the CAD software, the maxillary resection area and cutting planes and the fibular cutting planes and angles are determined. Once the resection area is decided, the necessary faces are extracted using a Boolean modifier. These superficial faces are united to fit the surface of the bones and thickened to stabilize the solids. Not only the cutting guides for the fibula and maxilla but also the location arrangement of the transferred bone segments is defined by thickening the superficial faces. The CAD design is recorded as .stl files and three-dimensionally (3-D) printed as actual surgical guides. To check the accuracy of the guides, model surgery using 3-D-printed facial and fibular models is performed. These methods may be used to assist surgeons where commercial guides are not available.

Methods for designing a computer-aided design/computer-aided manufacturing (CAD/CAM) surgical guide are shown. Cutting planes are separated, united, and thickened to easily visualize the necessary bone transfer. These designs can be three-dimensional printed and checked for accuracy.

내부 접근을 사용 하 여 상 악 재건을 위한 디자인 CAD/CAM 수술 가이드

Computer-aided design/computer-assisted manufacturing (CAD/CAM) is now being evaluated as a preparative technique for maxillofacial surgery. Because this ...

Porcine As a Training Module for Head and Neck Microvascular Reconstruction

Live models that resemble surgical conditions of humans are needed for training free-flap harvesting and anastomosis. Animal models for training purposes have been available for years in many surgical fields. We used the female (because they are easy to handle for the procedure) Yorkshire pigs for the head and neck reconstruction by harvesting the deep inferior epigastric artery perforator or the superior epigastric artery perforator flap. The anastomosis site (neck skin defect or tracheal wall defect) was prepared via the dissection of the common carotid artery and the internal jugular vein, in which 3.5&#215; loupe magnification was used for anastomosis as we use on human cases in real life. This procedure demonstrates a new training method using a reliable learning model and provides a detailed anatomy in a live scenario. We focused on the ischemia time, harvesting, vessel anastomosis, and designing the flap to fit the defect site. This model improves tissue handling and with the use of proper instruments can be repeated many times so that the surgeon is fully confident before starting the surgery on humans.

Here we present a protocol for the use of the pig superior epigastric artery perforator flap as a learning module for head and neck microvascular reconstruction.

돼지 머리와 목 Microvascular 재건을 위한 훈련 모듈

Live models that resemble surgical conditions of humans are needed for training free-flap harvesting and anastomosis. Animal models for training purposes ...

A Training and Testing System for Performing Vascular Reconstruction In Vitro

Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has yet to be developed. In this study, we introduce an in vitro training and testing system using a magnetic anchoring technique with which a trainee can practice manual vascular reconstruction individually. Additionally, this system can also be used to test the quality of the reconstruction. The described system includes a vascular reconstruction training machine, magnetic tractors, and a magnetic suture puller. In this manuscript, we detail an end-to-end vein anastomosis using porcine right and left iliac veins. To identify the potential damage caused by a magnetic suture puller on the suture, we created three groups with six segments of 4-0 polypropylene sutures each: a control group with no intervention on the polypropylene suture, a group in which the polypropylene suture is manually pulled with sterile gloves 20x, and a magnetic puller group in which the magnetic puller pulled the polypropylene suture 20x. These groups were tested by light microscopy and breaking strength tests, and the effect of reconstruction was assessed. In the light microscopy test, the control group was less likely to be damaged (p &#60; 0.05) and the number of damaged points of the manual group and magnetic puller group were similar (p &#62; 0.05). The results of the breaking strength test were compared across groups and no significant difference was observed (p &#62; 0.05). The end-to-end anastomosis of the porcine iliac veins was successfully performed using this training system, and the reconstructed veins could undergo 2.0 kPa perfusion pressure. Using this training and testing system the trainee can practice manual vascular reconstruction in vitro individually with the aid of magnetic tractors and a magnetic suture puller, and the quality of the reconstruction can be tested.

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.

체외에서 혈관 재건을 수행하기 위한 교육 및 테스트 시스템

Manual vascular reconstruction training is essential for a beginner surgeon. However, an optimal training system for vascular reconstruction in vitro has ...

Technical Detail for Robot Assisted Pancreaticoduodenectomy

Since its first report in 2003, robotic pancreaticoduodenectomy (RPD) has gained popularity among pancreatic surgeons. Inherent advantages of the robotic platform, including three-dimensional vision, wristed instruments, and improved ergonomics, allow the surgeon to recapitulate the principles of open pancreatoduodenectomy allowing safe oncologic dissection, hemostasis, and meticulous reconstruction. Over the course of the past decade, significant strides have been achieved in outlining the safety, feasibility, and learning curve of the robotic Whipple. When performed by high volume pancreatic surgeons experienced in RPD, recent comparative effectiveness studies show potential advantages compared to the open technique, including reductions in hospital stay and morbidity. National data also show reductions in conversion rates compared to its laparoscopic counterpart. Although long-term oncologic data are still needed, short-term oncologic surrogates of margin resection and lymph node harvest suggest no compromise in oncologic outcomes. As pancreatic surgeons increasingly integrate robotics into their practice, proficiency-based training and credentialing will be necessary for the safe application and dissemination of RPD. Here, we provide the detailed steps of a robotic pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.&#160;

The following manuscript details a stepwise approach to the robot-assisted pancreaticoduodenectomy performed at the University of Pittsburgh Medical Center.

로봇 보조 췌장췌장 절제술에 대한 기술적 세부 사항

Since its first report in 2003, robotic pancreaticoduodenectomy (RPD) has gained popularity among pancreatic surgeons. Inherent advantages of the robotic ...

A Postoperative Evaluation Guideline for Computer-Assisted Reconstruction of the Mandible

Valid comparisons of postoperative accuracy results in computer-assisted reconstruction of the mandible are difficult due to heterogeneity in imaging modalities, mandibular defect classification, and evaluation methodologies between studies. This guideline uses a step-by-step approach guiding the process of imaging, classification of mandibular defects and volume assessment of three-dimensional (3D) models, after which a legitimized quantitative accuracy evaluation method can be performed between the postoperative clinical situation and the preoperative virtual plan. The condyles and the vertical and horizontal corners of the mandible are used as bony landmarks to define virtual lines in the computer-assisted surgery (CAS) software. Between these lines the axial, coronal, and both sagittal mandibular angles are calculated on both pre- and postoperative 3D models of the (neo)mandible and subsequently the deviations are calculated. By superimposing the postoperative 3D model to the preoperative virtually planned 3D model, which is fixed to the XYZ axis, the deviation between pre- and postoperative virtually planned dental implant positions can be calculated. This protocol continues and specifies an earlier publication of this evaluation guideline.

Here, we propose a practical, feasible and reproducible evaluation guideline for computer-assisted reconstruction of the mandible in order to create uniformity between studies regarding postoperative accuracy evaluation. This protocol continues and specifies an earlier publication of this evaluation guideline.

하악골의 컴퓨터 지원 재건을 위한 수술 후 평가 지침

Valid comparisons of postoperative accuracy results in computer-assisted reconstruction of the mandible are difficult due to heterogeneity in imaging ...

Therapy Interventions for Upper Limb Amputees Undergoing Selective Nerve Transfers

Targeted Muscle Reinnervation (TMR) improves the biological control interface for myoelectric prostheses after above-elbow amputation. Selective activation of muscle units is made possible by surgically re-routing nerves, yielding a high number of independent myoelectric control signals. However, this intervention requires careful patient selection and specific rehabilitation therapy. Here a rehabilitation protocol is presented for high-level upper limb amputees undergoing TMR, based on an expert Delphi study. Interventions before surgery include detailed patient assessment and general measures for pain control, muscle endurance and strength, balance, and range of motion of the remaining joints. After surgery, additional therapeutic interventions focus on edema control and scar treatment and the selective activation of cortical areas responsible for upper limb control. Following successful&#160;reinnervation of target muscles, surface electromyographic (sEMG) biofeedback is used to train the activation of the novel muscular units. Later on, a table-top prosthesis may provide the first experience of prosthetic control. After fitting the actual prosthesis, training includes repetitive drills without objects, object manipulation, and finally, activities of daily living. Ultimately, regular patient appointments and functional assessments allow tracking prosthetic function and enabling early interventions if malfunctioning.

This work presents a protocol to enhance prosthetic function after selective nerve transfer surgery. Rehabilitation interventions include patient information and selection, support of wound healing, cortical re-activation of sensory-motor areas of the upper limb, training of selective muscle activation, prosthetic handling in daily life, and regular follow-up assessments.

Targeted Muscle Reinnervation (TMR) improves the biological control interface for myoelectric prostheses after above-elbow amputation. Selective ...

Management of the Uncinate Process in No-Touch Laparoscopic Pancreaticoduodenectomy

Laparoscopic pancreatoduodenectomy (LPD) is a demanding abdominal operation that necessitates meticulous surgical skills and teamwork. The management of the pancreatic uncinate process is one of the most important and difficult processes in LPD because of its deep anatomical location and difficult exposure. Complete resection of the uncinate process and mesopancreas has become the cornerstone of LPD. In particular, it is even more difficult to avoid positive surgical margins and incomplete lymph node dissection when the tumor is located in the uncinate process. No-touch LPD, which is an ideal oncological operation process fitting the "tumor-free" principle, has been reported by our group previously. This article introduces the management of the uncinate process in no-touch LPD. Based on the multi-angle arterial approach, in this protocol, the median-anterior and left-posterior approaches to the SMA are used to correctly deal with the important vascular structure, the inferior pancreaticoduodenal artery (IPDA), in order to ensure the safe and complete excision of the uncinate process and mesopancreas. For the achievement of the no-touch isolation technique in LPD, the pancreatic head and the blood supply to the duodenal region must be severed at the very early stage of the operation; after that, the tumor can be isolated intact, resection can be performed in situ, and finally, the tissue can be removed en bloc. This paper aims to show the distinctive ways to manage the uncinate process in no-touch LPD and investigate the viability and safety of this approach. Moreover, the technique may increase the R0 resection rate.

Complete resection of the uncinate process and mesopancreas is one of the most important and difficult processes in laparoscopic pancreatoduodenectomy (LPD). This article presents a method for managing the uncinate process in no-touch LPD using the median-anterior and left-posterior approaches to the superior mesenteric artery (SMA).

노터치 복강경 췌장 십이지장 절제술의 Uncinate 과정 관리

Laparoscopic pancreatoduodenectomy (LPD) is a demanding abdominal operation that necessitates meticulous surgical skills and teamwork. The management of ...

Digital Model for Horizontal 3D Reconstruction of Pulmonary Nodules

To reconstruct the three-dimensional digital model of the pulmonary nodules, implement the Three-Dimensional Lung Horizon Function in the MATLAB workspace. Then open the Graphical User Interface, or GUI, to check the horizontal three-dimensional model. Drag the scroll bar on the GUI with the mouse to observe the continuous three-dimensional lung structure, allowing for the clear visualization of various types of pulmonary nodules and their relative spatial relationships with the lung tissue.Use the Zoom function to observe the local features of the lesions and relevant three-dimensional structural output pictures. To measure the size of the nodules, use the Mark Pixel Coordinates button to calculate the distance between two points. The default color bar, jet colormap, displays values from low to high in a blue to red range.To reset the GUI, right click the color bar and select the standard gray colormap. If the filter window is not satisfactory, use the left mouse button to adjust the window level by dragging up and down in the middle of the figure. Drag left and right to adjust the window width to display the accurate filtering range on the color bar.The continuous three-dimensional reconstruction resulted in the axial view of the pulmonary nodules and the relationship between the nodules and the surrounding lung tissue.