Target Splenocyte Coating with Irrelevant and Cognate Peptides and Carboxyfluorescein Succinimidyl Ester (CFSE) Labeling
5:44
CFSE-Labeling Evaluation
6:50
CFSE-Labelled Target Cell Injection into Nave and T-Ag-Primed Recipients
7:31
Data Acquisition
8:21
Results: Representative Analyses of CD8+ T Cell-Mediated Cytotoxicity
9:36
Conclusion
Transcript
This assay can be employed to quantify T-cell-mediated cytotoxicity against two distinct peptide epitopes of tumor antigens simultaneously. The in vivo nature of this assay allows for cytotoxic function of T-cells to be measured within the intact
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We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy testing.