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DOI :
10.3791/59587-v
April 30th, 2019
Chapters
0:04
Title
0:36
Mouse Liver Tissue Collection
1:19
Fixation, Paraffin Embedding, and Liver Block Sectioning
2:35
Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
4:08
Imaging and Quantification of Bile Ducts (BDs)
5:27
Results: Determining BD to PV Ratio
7:00
Conclusion
我们提出了一种相当简单和敏感的方法,用于准确定量小鼠肝脏中的胆管密度。该方法有助于确定遗传和环境修饰剂的影响,以及潜在疗法在胆汁疾病小鼠模型中的有效性。
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