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7.6K Views
•
07:15 min
June 11th, 2019
DOI :
10.3791/59602-v
Chapters
0:04
Title
0:59
Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
2:10
LG Single-cell Suspension Preparation
4:46
Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
5:45
Results: Representative MEC and Pericyte Characterization and Isolation
6:38
Conclusion
Transcript
该协议是重要的,因为它允许分离和隔离两个光滑的肌肉细胞系,肌皮细胞和心状体。此方法通过细胞表面标记结合平滑肌肉细胞的遗传标记,以净化肌皮细胞和心状细胞的种群。该协议允许比较健康和患病的脑皮细胞的功能和再生能力,并处理这些细胞的基因表达研究。
乳腺细胞存在于其他外分泌腺体中,如乳腺、唾液和胰腺,这使得通过分离任何其他组织的脑皮细胞和周利特来适应此协议。要标记α平滑肌肉行为素或SMA表达细胞,注射三至四周大的塔莫西芬-可吸收阿尔法SMA驱动记者小鼠与100微升的塔莫西芬每20克体重
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Summary
泪腺 (LG) 有两种细胞类型表达α平滑肌肌动蛋白 (αSMA): 肌上皮细胞 (Mec) 和周周细胞。Mec 是外胚层的起源, 发现在许多腺体组织, 而周围的血管平滑肌细胞的内皮起源。该协议将 Mec 和 pericytes 与小鼠 Lg 隔离开来。
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