Results: Representative Site-Specific Inflammatory mRNA and Protein Detection
7:40
Conclusion
Transcript
Our protocol is an easy-to-use and inexpensive epidermal-dermal separation technique for determining the site-specific production of inflammatory mediators and neurotrophins during skin inflammation. The main advantage of this technique is that en
Sign in or start your free trial to access this content
Presented here is a protocol for the separation of epidermis from dermis to evaluate inflammatory mediator production. Following inflammation, rat hind paw epidermis is separated from the dermis by thermolysin at 4 °C. The epidermis is then used for mRNA analysis by RT-PCR and protein evaluation by western blot and immunohistochemistry.