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DOI :
10.3791/59709-v
•
6:25 min
July 10th, 2019
Chapters
0:04
Title
0:58
Preparation of RNA Dilution Series
1:22
Real-time PCR
3:33
Data Analysis
4:28
Results: Pan-lyssavirus RT-PCR
5:38
Conclusion
这种使用dsDNA间染的实时RT-PCR适用于诊断裂解病毒感染。该方法从从狂犬病疑似前身或死后样本中提取的RNA开始,详细说明主混合物制备、RNA添加、实时机器的设置以及结果的正确解释。
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