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9.1K Views
•
10:00 min
July 11th, 2019
DOI :
10.3791/59720-v
Chapters
0:04
Title
0:46
Protocol 1: Climbing Assay
1:41
Protocol 3: Histology Analysis
4:32
Protocol 4: Gene Mapping of Recessive Mutant Phenotype
6:17
Protocol 5: DNA Sequencing and Analysis
7:08
Protocol 6: Further Analysis of Candidate Gene Function
7:46
Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
9:11
Conclusion
Transcript
该协议的主要目标是利用前瞻遗传学的力量来识别基因,当调节不良导致神经退化时。这项技术的主要优点是,它为识别神经保护基因提供了一种公正而直接的方法,在哺乳动物模型中可能更难执行。该技术可用于识别与神经退化过程有关的新基因,该基因与许多疾病(如阿尔茨海默氏症和帕金森氏症)有关。
除了识别神经保护基因外,前向遗传方法还可用于发现其他生物过程(如神经元功能和传输)中涉及的基因。首先,从属于ENU变异集合的果蝇突变集合中收集同源苍蝇,这些变异体映射到第二染色体。将10至12天的苍蝇翻转到
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Summary
我们提出了一个协议,使用前向遗传方法筛选在果蝇黑色素细胞中表现出神经退化的突变体。它结合了爬升测定、组织分析、基因映射和DNA测序,最终鉴定出与神经保护过程相关的新基因。
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