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DOI :
10.3791/59724-v
•
7:22 min
May 14th, 2019
Chapters
0:04
Title
0:35
Tools and Solutions for Injections
1:30
Preparation of the Injection Station
2:28
Preparation of the Fish for Intrathoracic Injection
3:04
Microinjection into the Pericardium
4:07
Results: Validation and Analysis of the Intrathoracic Injection Method
6:35
Conclusion
この方法は、0.5 −3μ l の溶液を成体ゼブラフィッシュの胸郭に注入することに依存する。この手技は、タンパク質や化学化合物を、器官を傷つけることなく、ゼブラフィッシュの心臓の近くに効率的に供給します。アプローチは、心臓の様々な組織に外因性因子の効果をテストするのに適しています.
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