Bone Marrow, Liver, Thymus-Humanized (hu-BLT) Mouse Generation
3:43
Human Immune Cell Level Evaluation and Fresh Fecal Sample Collection
5:20
Fecal Transplant
6:05
Results: Representative Double Humanized BLT-Mouse Gut Microbiome Analyses
7:02
Conclusion
Transcript
Our protocol is significant, because it can be use to reproducibly create mice with both a human gut microbiome and a human immune system. We call these mice, double humanized mice. The double humanized mouse model can be used for both basic and t
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We describe a novel method for generating double humanized BLT-mice that feature a functional human immune system and a stable engrafted human-like gut microbiome. This protocol can be followed without the need for germ-free mice or gnotobiotic facilities.