Allogenic Tumor Mouse Model and Neutrophil Adoptive Transfer
2:32
Tumor Growth Evaluation
3:54
Tumor-Associated Neutrophil (TAN) Isolation
6:04
In Vitro FK866-TAN Inhibition
6:44
Results: Representative Analyses of FK866 Treatment on Neutrophil Angiogenic Properties and Tumor Growth
7:32
Conclusion
Transcript
Our protocol provides a novel tool for evaluating the angiogenic potential of tumor-associated primary neutrophils in vivo without the need to use artificial neutrophil cell line models. Direct inhibition of nicotinamide phosphoribosyltransferase,
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Here, we show therapeutic potential of anti-angiogenic tumor-associated neutrophils after their transfer into tumor-bearing mice. This protocol can be used to manipulate neutrophil activity ex vivo and to subsequently evaluate their functionality in vivo in developing tumors. It is an appropriate model for studying potential neutrophil-based immunotherapies.