Intravenous Injection of Specific and Nonspecific Antibody Agents
2:27
Collection and Preparation of Target Tumor Tissues
3:53
Ex Vivo Staining Protocol
6:07
Results: IVIL Shows Varying Biodistribution Patterns of Anti-B7-H3 and Anti-Netrin-1 Antibodies in Murine Mammary Tumors In Vivo Compared to Respective Control Antibodies
7:50
Conclusion
Transcript
This protocol for in vivo immunofluorescence localization, or IVIL, assesses the in vivo biodistribution of diagnostic and therapeutic antibodies or antibody-based agents for cancer research, detection, and treatment. In contrast to conventional i
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The in vivo immunofluorescence localization (IVIL) method can be used to examine in vivo biodistribution of antibodies and antibody conjugates for oncological purposes in living organisms using a combination of in vivo tumor targeting and ex vivo immunostaining methods.