July 16th, 2019
•Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.
Related Videos
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
Following Cell-fate in E. coli After Infection by Phage Lambda
Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Listeria monocytogenes Infection of the Brain
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved