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DOI :
10.3791/59823-v
•
9:00 min
June 16th, 2019
Chapters
0:04
Title
1:11
Pluripotent Stem Cell Colony Formation
2:45
Hemogenic Induction and Isolation of Hemogenic Endothelium
4:08
Induction of Endothelial-to-hematopoietic Transition (EHT)
5:00
Flow Cytometry Analysis of Hematopoietic Cells
6:27
Colony Forming Unit Assay of Hematopoietic Cells
7:30
Results: Hemogenic Induction
8:19
Conclusion
여기서, 우리는 피더-및 제노프리 조건에서 인간 다능성 줄기 세포로부터 혈생 내피를 정밀하게 형성하는 프로토콜을 제시한다. 이 방법은 치료 화합물에 대한 질병 모델링 및 스크리닝에 광범위한 응용 프로그램을 가질 것이다.
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