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06:34 min
January 21st, 2020
DOI :
10.3791/59864-v
Chapters
0:04
Title
1:15
Installation and Testing
2:56
Identifying Novel MicroRNAs (miRNAs)
3:33
Reference Index Building
4:42
Results: Representative miRDP2 Performance
5:45
Conclusion
Transcript
miRDeep2 可用于准确识别植物微RNA,这些微RNA是植物开发中重要的转录调节器,对于应对环境挑战至关重要。miRDeep2 需要明显较短的运行时间,在灵敏度和准确性方面表现出出色的性能,特别是用于预测具有大基因组的植物中的微RNA。假阳性和长处理时间是植物微RNA注释的挑战。
通过添加新的场内策略、彻底改革评分算法和集成严格的标准,miRDeep2 可以克服这些问题。高置信度微RNA注释对于发现微RNA在调节基因组不同功能中的作用至关重要。分步指导对首次使用微RNA注释
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Summary
生物信息学管道,即miRDeep-P2(简称miRDP2),具有更新的植物miRNA标准和经过全面检查的算法,可以准确有效地分析植物中的微RNA转录,特别是对于具有复杂和大型基因组的物种。
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