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JoVE Journal

Genetics

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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Transcript

miRDeep2 can be used to accurately identify plant microRNAs that are important transcriptional regulators in plant development and are crucial for responding to environmental challenges. miRDeep2 requires a markedly short running time and exhibits

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A bioinformatics pipeline, namely miRDeep-P2 (miRDP2 for short), with updated plant miRNA criteria and an overhauled algorithm, could accurately and efficiently analyze microRNA transcriptomes in plants, especially for species with complex and large genomes.

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