Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
August 14th, 2019
•The characterization of circulating tumor cells (CTCs) is a popular topic in translational research. This protocol describes a semi-automatic immunofluorescence (IF) assay for PD-L1 characterization and enumeration of CTCs in non-small cell lung cancer (NSCLC) patient samples.
Tags
Related Videos
Lung Tumor Cell Recruitment Assay
Capture and Release of Viable Circulating Tumor Cells from Blood
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization
The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues
Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients
Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression
Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved