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11.8K Views
•
14:27 min
August 11th, 2019
DOI :
10.3791/59879-v
Chapters
0:00
Title
1:00
Animal Preparation: Draining of Cerebrospinal Fluid
2:35
Animal Preparation: Craniotomy
4:22
Data Acquisition: In Vivo
6:06
In Vitro Setup
7:10
Brain Slicing
11:00
Data Acquisition: In Vitro
12:48
Results
13:43
Conclusion
Transcript
长期突触可塑性,特别是长期增效和长期抑郁症,已经沿着海马体的横向被广泛研究。它也沿纵向方向观察。然而,当涉及到纵向方向时,对海马体的纵向CA1轴在突触可塑性方面的关注很少。
我们实验室的一份先前出版物显示,海马体的CA1金字塔神经元形成关联连接,可以支持突触可塑性。为了进一步理解此协议,建议您参考上一个出版物,并给出以下引用。注射聚氨酯麻醉小鼠。
将完全麻醉的鼠标放在设置为 37 摄氏度的加热垫上。涂抹眼凝胶滋润眼睛。用眼夹牢牢固定鼠标的头骨。
眼夹为实
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Summary
我们在纵向海马脑切片中使用记录和刺激电极,并在体内背海马区中纵向定位记录和刺激电极,以唤起细胞外的显微电位并演示沿纵向间层 CA1 的长期突触可塑性。
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