Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
8.9K Views
•
09:15 min
August 16th, 2019
DOI :
10.3791/60018-v
Chapters
0:05
Title
0:40
Egg Collection
1:53
Scanning Electron Microscopy (SEM) Preparation: Embryo Chemical Drying
4:18
SEM Preparation: Eggshell Air-Drying
4:53
SEM Preparation: Fungal Culture Critical Point Drying
7:12
Results: Representative Scanning Electron Microscopic (SEM) Analysis of Painted Turtle Embryos and Eggshells
8:37
Conclusion
Transcript
我们有标准化的样品制备方案,用于成像彩龟胚胎,硬蛋壳和真菌培养使用扫描电子显微镜。这些综合方法使用对已知协议的细微改变来处理三个微妙的组织,从而可以区分原始结构和处理伪影。与我展示这个程序的将是杰西卡·吉本斯,一个来自我实验室的研究生。
在筑巢季节从田间地场采集海龟蛋之前,请沿塑料盒两侧和盖子上打四到六个 0.25 厘米的孔,以便进行呼吸。在将湿润的紫土和泥炭苔床上用品混合物以一比一的比例填充包装盒之前。在筑巢地点,轻轻清除土壤,以发现鸡蛋,用稀释的碘药擦拭每个海龟蛋的表面,以
Sign in or start your free trial to access this content
Summary
在这里,我们提出了使用扫描电子显微镜(SEM)成像精细组织样本的详细处理方案。介绍了三种不同的处理方法,即六甲基二甲苯(HMDS)化学干燥、简单空气干燥和临界点干燥,分别用于制备硬质蛋壳、早期发育阶段的胚胎和真菌培养。
Explore More Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved