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Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

DOI :

10.3791/60045-v

11:27 min

September 18th, 2019

September 18th, 2019

8,860 Views

1Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)

Presented here is a protocol for performing single-molecule Förster resonance energy transfer to study HJ resolution. Two-color alternating excitation is used for determining the dissociation constants. Single-color time lapse smFRET is then applied in real-time cleavage assays to obtain the dwell time distribution prior to HJ resolution.

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Single Molecule F rster Resonance Energy Transfer

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