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6.9K Views
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10:03 min
November 5th, 2019
DOI :
10.3791/60047-v
Chapters
0:05
Title
1:48
Preparation of Bacteria for In Vivo Infection Studies
3:09
Intra-cisternal Injection of Mice
5:07
Evaluation of Colony Forming Units (CFU) Counts in Peripheral Organs
6:39
Preparation of Brain Tissues for CFU Count
8:05
Results: Survival of Mice Following Intracisternal Injection of Wild-type and Mutant N. meningitidis
9:28
Conclusion
Transcript
脑膜炎是全世界人口中败血症和脑膜炎最常见的病因之一,细菌仅限于人类宿主。在这项实验协议中,我们将描述在基于细菌的病毒接种的小鼠模型中诱导脑膜炎球菌脑膜炎的定制程序。这个穆林模型再现了脑膜炎球菌性脑膜炎作为一种严重人类疾病的特征病理事件。
因此,它代表了一个有用的工具,以评估宿主-病原体相互作用,并分析导致这种致命疾病的病原体机制。感染模型不仅有助于评估防止细菌直接复制到CSF的创新治疗策略,而且有助于分析可能被动免疫疗法对人类病原体的疗效。该技术要求训练阶段,以确保不仅在治疗性
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Summary
在这里,我们描述了一种通过成年小鼠的间感染途径诱导脑膜炎球菌性脑膜炎的方法。提出了从接种制备到间感染的脑膜炎球菌感染的一步一步方案;然后记录动物的生存,并评估鼠组织中的细菌负荷。
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