Chemical Synthesis of the Hydrazine Derivative of a Fc-III Peptide
2:31
Cleavage of the SUMO Tag
4:09
Assembling of DCAF1 by Native Chemical Ligation
7:06
Mass Spectrometry and ELISA Assay
8:19
Results: Native Chemical Ligation and ELISA Assay
9:03
Conclusion
Transcript
This might serve to help answer key questions about how to synthesize DCAF molecules with the targeted antibody neutralization. This technique provides the simplest way to synthesize of the DCAF molecules with high productivity. The world decide D
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Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) is novel for the elimination of harmful antibodies. Here, we describe a detailed protocol for the synthesis of DCAF1 molecule, which can selectively block 4G2 antibody to eliminate antibody dependent enhancement effect during Dengue virus infection.