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DOI :
10.3791/60147-v
February 27th, 2020
Chapters
0:05
Introduction
1:24
Plating and Differentiation Induction of HC11 Cells
3:36
3D Growth and Differentiation Induction of EpH4 Cells in EHS Matrix
6:24
Western Blotting for β-Casein
7:42
Results: Assessment of HC11 and EpH4 Cell Differentiation
8:49
Conclusion
我们描述了两个乳房上皮线HC11和EpH4的分化诱导技术。虽然两者都需要胎儿小牛血清、胰岛素和乳素来产生牛奶蛋白,但EpH4细胞可以在三维培养中完全分化成乳腺球。这些互补模型对于分化和肿瘤的信号转导研究非常有用。
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