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DOI :
10.3791/60182-v
September 27th, 2019
Chapters
0:05
Title
1:30
Extraction and Derivatization of CoA (Coenzyme A) in Cultured Cells
3:32
Extraction & Derivatization of CoA in Tissues
5:31
Sample Clean-up with Solid Phase Extraction Column
6:47
Results: HPLC Profiles and CoA-bimane Standard Curves
7:45
Conclusion
该方法描述了来自培养细胞和动物组织的样品制备,在样品中提取和衍生辅酶A,然后高压液相色谱用于纯化和定量衍生辅酶A吸光或荧光检测。
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