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DOI :
10.3791/60233-v
September 18th, 2019
Chapters
0:04
Title
0:40
Embryo Collection
3:21
Larval Collection
4:05
Needle Preparation and Specimen Injection
5:43
Embryo Imaging
7:27
Results: Representative Blood-Brain Barrier (BBB) Formation Analysis
8:22
Conclusion
6:17
Larval Dissection and Imaging
血脑屏障完整性对神经系统功能至关重要。在果蝇黑色素仪中,血脑屏障是由胚胎发育晚期的胶质细胞形成的。该协议描述了在D.melanogaster胚胎和第三星幼虫中进行血脑屏障形成和维护的测定方法。
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