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DOI :
10.3791/60253-v
October 27th, 2019
Chapters
0:05
Title
1:05
Spawning Agent Administration
2:08
B. gauderio Embryo Fertilization
4:25
Single Cell Microinjection
6:19
Results: Representative CRISPR/Cas9 Induced Mutations in B. gauderio and B. brachyistius
7:17
Conclusion
在这里,提出了一个协议,以生产和后CRISPR/Cas9基因组敲除电鱼。详细列出了运动和生物形态所需的分子生物学、育种和饲养要求,以及生产 Cas9 诱导 Indel F0幼虫的注射技术。
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