Isolering og kultur av nervus, Trochlear, og spinal motor neurons fra prenatal ISLMN: GFP transgene mus
November 12th, 2019
•Ryosuke Fujiki1,2,3,4,9, Joun Y. Lee1,2,10, Julie A. Jurgens1,2,3,7, Mary C. Whitman2,5,6, Elizabeth C. Engle1,2,3,4,5,6,7,8
1Department of Neurology, Boston Children's Hospital, 2FM Kirby Neurobiology Center, Boston Children's Hospital, 3Department of Neurology, Harvard Medical School, 4Medical Genetics Training Program, Harvard Medical School, 5Department of Ophthalmology, Boston Children's Hospital, 6Department of Ophthalmology, Harvard Medical School, 7Broad Institute of M.I.T. and Harvard, 8Howard Hughes Medical Institute, 9Department of Neurology, Kokura Memorial Hospital, 10Department of Genetics, Albert Einstein College of Medicine
Dette arbeidet presenterer en protokoll for å gi homogene cellekulturer av primære nervus, trochlear, og spinal motor neurons. Disse kulturene kan brukes til sammenlignende analyser av morfologiske, cellulære, molekylære, og elektrofysiologisk karakteristikker av øye og spinal motor neurons.
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