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JoVE Journal

Developmental Biology

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Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

5.8K Views

08:14 min

March 17th, 2020

March 17th, 2020

5,799 Views

0:05

Introduction

0:52

Left Anterior Descending Artery (LAD) Ligation-Induced Coronary Artery Occlusion (Myocardial Infarction)

2:58

Passive Clear Lipid-Exchanged Acrylamide-Hybridized Rigid Imaging Compatible Tissue Hydrogel (CLARITY)

5:53

Single-Photon Confocal Microscopy Cleared Mouse Heart Imaging

6:28

Results: Representative Neonatal Mouse Visualization

7:46

Conclusion

Transcript

The endogenous heart regeneration is a dynamic process that involves multiple cell types working together in concert to mount a robust regenerative response. Peering into the regenerating tissue via 3D imaging of the cleared heart in response to i

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Cardiomyocyte proliferation following injury is a dynamic process that requires a symphony of extracellular cues from non-myocyte cell populations. Utilizing lineage tracing, passive CLARITY, and three-dimensional whole-mount confocal microscopy techniques, we can analyze the influence of a variety of cell types on cardiac repair and regeneration.

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