DPP affect cell fate by binding receptors and sending a signal intracellularly to affect transcription. If DPP is produced but not released, it doesn't have access to receptors and therefore cannot perform its roles. This technique makes it possib
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The timing of exposure to ligands may impact their developmental consequences. Here we show how to image release of a Drosophila bone morphogenetic protein (BMP) called Dpp from cells of the wing disc.