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06:27 min
October 20th, 2020
DOI :
10.3791/60569-v
Chapters
0:04
Introduction
0:33
Polybubble Formation
1:55
Cargo Centering
2:40
Small Molecule Cargo Release and Near Infrared (NIR) Polybubble Activation
4:25
Results: Representative NIR Sensitive, Core-Shell Vaccine Delivery Platform Fabrication
5:57
Conclusion
Transcript
该协议可以通过使用多泡帮助保持货物稳定性,这既是小分子,也可以是蛋白质,从而减少与溶剂的碳相互作用。这种新技术可用于提高发展中国家的疫苗覆盖率,因为由于运输和储存设施效率低下,疫苗的功能往往受到损害。要制造多泡,首先使用一毫升转移移液器将 800 微升 10%CMC 添加到 0.92 毫升玻璃瓶中。
合成PCLTA,在200微升DCM中混合1000克每毫升14千吨PCL。合成PLGADA,将每毫升1000克5千达吨PLGADA混合成200微升氯仿。以0.005比1的比例将光启动
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Summary
本文介绍了用于生产新型疫苗输送平台"多泡"的协议,以实现延迟突发释放。聚酯(乳酸共糖酸)和多丙酮用于形成多泡,小分子和抗原用作货物。
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