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JoVE Journal

Genetics

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LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

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12:18 min

February 1st, 2020

February 1st, 2020

5,610 Views

0:00

Introduction

2:39

Isolation of Osteosarcoma-derived EVs

4:22

Characterization of OS-EVs

4:37

Treatment of MSCs with OS-EVs

5:08

LINE-1 Methylation Assay

7:57

Fragment Data Analysis

9:59

Results

11:25

Conclusion

Transcript

DNA methylation is one of the most studied epigenetic signatures. Many cancers are characterized by changes in DNA methylation levels, which are associated with aberrant gene expression and other genomic abnormalities. Long interspersed nuclear el

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Described here is the use of a methylation-specific probe amplification method to analyze methylation levels of LINE-1 elements in mesenchymal stem cells treated with osteosarcoma-derived extracellular vesicles. Ultracentrifugation, a popular procedure for separating extracellular vesicles from fetal bovine serum, is also demonstrated.

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