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8.3K Views
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08:15 min
January 31st, 2020
DOI :
10.3791/60723-v
Chapters
0:04
Title
0:50
Cord Blood CCR5Δ32 Genetic Variant Screening
1:50
Intravaginal HIV Exposure
3:20
RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
4:55
Application of Combination Antiretroviral Therapy (cART)-Containing Chow
5:42
Results: Representative HIV infection and Treatment DATA
7:13
Conclusion
Transcript
建立这种人性化的小鼠模型有助于临床前HIV研究。例如,可以评估不同的病毒株和新的艾滋病毒干预措施。这些协议可用于评估干细胞捐献者CCR5变异状态,有效感染艾滋病病毒的人类小鼠,并量化小鼠血浆病毒载量。
RNA定量协议可以很容易地适应,以量化任何病毒RNA选择序列,在血浆样品中可检测到。用于CCR5增量32变种脐带血样的基因筛选,用含有200微摩尔dNTP混合物的11.25微升,孵育1.25微升非颗粒流,每微升0.01单位高保真DNA聚合酶,以及表中详述的向前和反向等素。将无核酸
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Summary
我们开发了一种基于干细胞移植、阴道内人体免疫缺陷病毒暴露和滴数字PCR RNA的人性化和人体免疫缺陷病毒感染NOG小鼠模型的生成和评估方案量化。
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