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DOI :
10.3791/60734-v
March 25th, 2020
Chapters
0:04
Introduction
1:02
Methyl-Beta-Cyclodextrin (MBCD) Cholesterol-Enriching Solution Preparation
2:12
Cholesterol-Saturated Tissue Enrichment
5:07
Cholesterol Liposome-Enriched Phospholipid-Based Dispersion Preparation
6:06
Xenopus Oocyte Cholesterol Enrichment
7:37
Results: Representative Effects of Rat Cerebral Artery and Xenopus Oocyte Cholesterol Enrichment
9:22
Conclusion
提出了两种胆固醇富集方法:应用富含胆固醇的环糊精来丰富哺乳动物组织和细胞,以及利用富含胆固醇的磷脂型分散物(脂质体)来丰富Xenopus卵母细胞。这些方法有助于确定胆固醇水平升高对分子、细胞和器官功能的影响。
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