Results: Representative PA and PAA Surfacing and EC Quantification
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Conclusion
Transcript
This method allows each pharyngeal arch to be compartmentalized. Endothelial cell numbers can then be quantified in each arch as a means to study pharyngeal arch artery development. The main advantages of this approach are the ability to visualize
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Here, we describe a protocol to visualize and analyze the pharyngeal arch arteries 3, 4, and 6 of mouse embryos using whole-mount immunofluorescence, tissue clearing, confocal microscopy, and 3D reconstruction.