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DOI :
10.3791/60808-v
April 2nd, 2020
Chapters
0:05
Introduction
0:35
In Vitro Fertilization
2:23
Embryo Cryopreservation
3:13
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
4:19
CRISPR Electroporation
5:09
Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
6:09
Conclusion
在这里,我们提出了一种用于冷冻保存单细胞胚胎的改良方法,以及一种用于使用冷冻解冻胚胎和电穿孔以有效生成转基因小鼠的协议。
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