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DOI :
10.3791/60808-v
April 2nd, 2020
Chapters
0:05
Introduction
0:35
In Vitro Fertilization
2:23
Embryo Cryopreservation
3:13
Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
4:19
CRISPR Electroporation
5:09
Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
6:09
Conclusion
ここでは、1細胞胚の凍結保存のための改変方法と、遺伝子組換えマウスの効率的な生成のための凍結解凍胚とエレクトロポレーションの使用を組み合わせたプロトコルを提示する。
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