Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/60843-v
May 24th, 2020
Chapters
0:04
Introduction
0:50
Human Pluripotent Stem Cell (hPSC) Maintenance
1:48
hPSC Differentiation (Day 0)
3:34
Neural Crest Cell (NCC) Induction (Day 1-10)
4:02
NC Spheroid Aggregation (Day 10)
5:14
NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
6:09
Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
7:38
Results: Representative NC Induction, Maintenance, and Expansion
9:43
Conclusion
이 프로토콜에서, 우리는 인간 만능 줄기 세포에서 포스트 갱글리오닉 교감 뉴런의 생성을위한 안정적이고 매우 효율적인 분화 전략을 설명합니다. 이 모델은 여러 자율 장애의 연구의 사용을 위해 뉴런을 사용할 수 있게 합니다.
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved