Markerless Gene Deletion par Floxed Cassette Allelic Exchange Mutagenesis in Chlamydia trachomatis

0:27

Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest

2:20

Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange

5:54

Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution

7:38

Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette

8:42

Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects

9:47

Conclusion