クラミジア・トラコマティスにおけるフロキセカセットアレリック交換変異誘発によるマーカーレス遺伝子欠失

0:27

Design and Assembly of pSUmC-4.0 with Homology Arms Specific to the Gene of Interest

2:20

Transformation of C. trachomatis with pSUmC 4.0 + Homology Arms for Gene Deletion by Allelic Exchange

5:54

Clonal Isolation of Green-only C. trachomatis Deletion Mutant Containing the loxP Flanked Selection Cassette by Limiting Dilution

7:38

Transformation of C. trachomatis FRAEM Mutant with pSU-Cre to Initiate Removal of loxP Flanked Selection Cassette

8:42

Results: FLAEM Enables Markerless Gene Deletion in C. trachomatis and alleviates Cassette-induced Polar Effects

9:47

Conclusion