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JoVE Journal

Biochemistry

This content is Open Access.

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

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08:00 min

April 1st, 2020

April 1st, 2020

6,658 Views

0:04

Introduction

0:30

Microfluidic Flow System Assembly

2:05

Microfluidic Flow System for in vivo FPOP

4:13

In vivo FPOP

5:52

Results: IV-FPOP Oxidative Modification of Proteins within C. elegans

7:37

Conclusion

Transcript

In vivo fast photochemical oxidation of proteins (IV-FPOP) is a hydroxyl radical protein footprinting technique that allows for mapping of protein structure in their native environment. This protocol describes the assembly and set-up of the IV-FPOP microfluidic flow system.

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