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DOI :
10.3791/60911-v
•
9:03 min
March 11th, 2020
Chapters
0:04
Introduction
0:39
In-Cell Fast Photochemical Oxidation of Proteins (IC-FPOP) System Assembly
2:53
Cell Preparation
3:44
IC-FPOP
5:40
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Analysis
6:21
Results: Representative IC-FPOP Characterization
8:14
Conclusion
ここでは、細胞内でのタンパク質の高速光化学酸化(IC-FPOP)と呼ぶタンパク質フットプリント技術を用いて、生細胞におけるタンパク質構造および相互作用部位を特徴付ける。
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