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10:01 min
April 8th, 2020
DOI :
10.3791/60935-v
Chapters
0:05
Introduction
0:45
Protein Expression and Preparation
3:38
Dye-modification of Elastin-like Proteins via SPAAC
4:44
THF Swelling Protocol
6:29
BuOH Extrusion Protocol
7:19
Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
9:22
Conclusion
Transcript
有效组装超分子结构,以最小的细胞设计和药物输送仍然具有挑战性。在这里,我们演示了基于两栖弹性蛋白蛋白产生超分子结构的高效方案。提出的装配方案可生成具有适应性物理化学特性的多功能超分子结构,如囊泡、纤维和共热物。
基于蛋白质膜的隔间演示相分离行为,并允许封装化学多样化的荧光货物分子。对于 F20E20-mEGFP 和 F20E20-mCherry 的表达,将主要表达区域性从隔夜前培养到 OD600 的 0.3。在 400 毫升无菌 LB 介质中孵育,并在 37 摄氏度和 200
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Summary
在有机溶剂和水性溶剂的界面上,定制的两栖乳素样蛋白质组装成复杂的超分子结构,如由环境参数触发的囊泡、纤维和焦质。所述装配协议产生具有可调特性的蛋白质膜型隔间 (PMBC),能够封装各种货物。
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